FastQCFastQC Report
Tue 21 Jan 2020
Ler.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24454018
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT249590.10206502669622636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86650.024.90304694
CGGGAAC108300.013.9676991
TTAATAC59950.013.8197693
GGGGGAT75400.013.5189431
CGCGTAT18750.013.1566281
CAGACGT55000.012.6940023
TTTAATA97250.012.4916812
CGTGTGC59800.012.1420887
GGGCCCT27600.011.4675278
ATTTAAT90000.011.325871
TTGAGGT276150.011.1410567
TAGGGCG28650.011.0472528
GACGTGT61050.010.9785955
GTTTTAT158650.010.8257071
GTTTAAT91650.010.8172571
TTTGAGG332000.010.26225956
ACGTGTG67650.010.1827226
TAATACA83850.010.1582294
GTTTTTT271950.09.9952521
GTTTTTA145300.09.9624191
Produced by FastQC (version 0.11.5)