FastQCFastQC Report
Tue 21 Jan 2020
Ler.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24634955
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64600.030.74775994
TAGGGCG26600.012.4336188
GGGGGAT63900.012.1011891
CGGGAAC130750.011.9706681
GTTTTAT155050.011.4765771
GTTTTTT289250.011.257051
CTTAAAC159050.010.6021921
CGCGTAT20000.010.4810141
TTTAATA98600.010.3936142
TTAAACG83850.010.2775472
CTTTTAT178050.010.1772021
TTAATAC56650.010.0318433
GTAGGGC31700.09.9924147
CTCGAAT166950.09.9609931
CTTAGAT108400.09.7547781
TTGAGGT275200.09.5635877
AGGGCGG37600.09.4155699
GTTTGAT329150.09.3971251
CTTTAAT141500.09.38229751
GTTTAAT96350.09.3793081
Produced by FastQC (version 0.11.5)