FastQCFastQC Report
Tue 21 Jan 2020
Ler.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24634955
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86650.020.91207394
CGCGTAT18600.015.0220871
CGGGAAC108850.013.519191
TTAATAC62450.012.0056433
TTTAATA98650.011.4237922
GGGGGAT76800.011.2176661
CTTAAAC160850.011.1752761
GTTTTAT160200.011.07527351
GTTTTTT262100.010.9092111
ATTTAAT91500.010.738741
TTGAGGT281000.010.4737637
TAGGGCG29600.010.0688598
CTTTTAT164900.09.8841511
GTTTTTA156200.09.8682241
GTTTTGT244150.09.7275931
AGTTTTG286500.09.6875423
CTCGAAT147650.09.682711
TGAGGTT301100.09.4961958
CGAAAAT130100.09.4855081
TTTGAGG335650.09.4205266
Produced by FastQC (version 0.11.5)