FastQCFastQC Report
Tue 21 Jan 2020
Ler.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24571735
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG59800.024.6457494
GGGGGAT68550.013.1189541
TAGGGCG31700.012.7881978
CGCGTAT21150.012.7781191
CGGGAAC134500.012.7489631
GTTTTAT157300.012.1155721
CGTGCAT50850.012.03933694
TTAAACG83750.011.7950662
GTAGGGC34450.011.63211257
AGGGCGG38100.011.3738469
GTTTTTT278350.011.316311
CTTAAAC160750.011.2468281
CCCCGCG31650.010.3878194
GTCGGTT70900.010.3838931
CTTTTAT174700.010.3487571
CTTAGAT109500.010.2553921
TAAACGT87000.09.961923
TTGAGGT285650.09.9178767
GTTTTTA156250.09.9007341
TTTGAGG321950.09.8995866
Produced by FastQC (version 0.11.5)