FastQCFastQC Report
Tue 21 Jan 2020
Ler.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLer.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24571735
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT249470.10152722223318784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86200.016.59397594
CGGGAAC112550.013.7255261
TTAATAC60350.013.7239493
GGGGGAT75000.012.5321761
TAGGGCG30350.012.4183168
CGCGTAT19200.012.3596261
TTTAATA96850.012.347242
CGTGCAT44500.011.7350594
GTTTTTT248200.011.4544921
CCCGCTA31100.011.2211487
CTTAAAC169000.010.9305051
ATTTAAT92050.010.8680361
GTTTTAT148200.010.6435831
GTAGGGC33900.010.5688427
TTGAGGT276300.010.356927
GTTTTTA147550.010.2489771
CTTTAAT136750.010.2077571
GTCGGTT69650.010.0877011
AGTTTTG286200.09.933633
CGCTAGG33300.09.9208879
Produced by FastQC (version 0.11.5)