FastQCFastQC Report
Tue 21 Jan 2020
Mp.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24844038
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG55900.036.1533194
GGGGGAT62850.013.7813051
CGGGAAC128900.013.4030511
CGTGCAT54500.011.55530494
GTTTTTT289600.011.320271
CTTAAAC159800.011.1027581
GTTTTAT160450.011.08680251
TAGGGCG29300.010.8074688
TTAAACG85250.010.7064232
TAAACGT83250.010.68394853
GTTTTTA163400.010.2881621
GGGGATA84750.010.2750652
TTTGAGG323250.09.8681276
TTGAGGT300050.09.8551397
CTTTTAT173600.09.8446221
GTTTGAT346600.09.7810511
CCCCGCG32900.09.74489894
GTTTACT130100.09.5926851
GTCGATT101700.09.5699131
CTTTAAT142850.09.5514661
Produced by FastQC (version 0.11.5)