FastQCFastQC Report
Tue 21 Jan 2020
Mp.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24844038
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT259370.10439929290077563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG76500.026.63720194
CGGGAAC106250.014.3565481
TAGGGCG30400.012.6972498
GGGGGAT74750.011.2608841
GTAGGGC34700.011.2578367
ATTTAAT84800.011.2425071
GTTTTTT251900.011.0217691
TTAATAC63900.010.9895833
CTTAAAC176750.010.8666521
TTTAATA99750.010.5832262
TTGAGGT291650.010.5400977
TAAACGT83300.010.4400293
CTTTTAT159650.010.4284361
TTAAACG95750.010.2482282
GGGCCCT34100.010.2285018
CTTTAAT150250.010.0284931
TTTGAGG334500.09.9962686
AGTTTTG300250.09.928423
CCCCGCG45550.09.92747394
CTCGAAT141950.09.8285841
Produced by FastQC (version 0.11.5)