FastQCFastQC Report
Tue 21 Jan 2020
Mp.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24576682
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG58100.035.8425994
CGGGAAC130250.013.8440331
GGGGGAT61700.013.8195961
TAGGGCG30550.013.42149458
GTAGGGC35700.011.6158567
CGTGCAT54800.011.49348794
GTTTTAT160350.011.245311
GGGCCCT37450.011.0730598
GTTTTTT282350.010.6769181
TTTAATA96200.010.6071442
ACCGTGC65150.010.3701018
GGGGATA79100.010.3672982
TTTGAGG312050.010.1235996
TTGAGGT278600.010.0680377
TTAATAC57050.010.0456693
CTTAAAC156350.09.8939491
AGGGCGG43100.09.8376999
GTTTAAT94750.09.736781
CCCCGCG32900.09.658318594
GGAAAAT241200.09.5621881
Produced by FastQC (version 0.11.5)