FastQCFastQC Report
Tue 21 Jan 2020
Mp.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24576682
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG75150.027.78104294
CGGGAAC110000.014.8965761
GGGGGAT70850.013.206651
TAGGGCG30200.012.7940388
TTAATAC55950.011.981163
GTAGGGC31400.011.7120777
CGCGAAC34600.010.89796451
TTAAACG89450.010.8768322
TTTAATA94950.010.5899832
CGCGTAT18150.010.5158331
GTTTTAT161750.010.5047341
CTTAAAC170750.010.441781
TAAACGT85750.010.2603973
GTTTTTT254950.010.0790721
CGCAAAC96200.09.96847251
TTGAGGT278150.09.9245797
CGTGCAT49850.09.84758994
ATTTAAT85250.09.7745131
CTTTAAT137300.09.6968781
GGAAAAT243600.09.6696261
Produced by FastQC (version 0.11.5)