FastQCFastQC Report
Tue 21 Jan 2020
Mp.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24590389
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG51550.033.0885994
GGGGGAT62650.018.3652781
CGGGAAC134850.014.2666091
CGTGCAT54350.014.01815794
GGGGATA83850.013.4997242
GTTTTAT160700.010.8701881
TTGAGGT284750.010.8133287
TTTGAGG318850.010.6064956
CTTAAAC166050.010.5480131
GTTTTTT291900.010.5005841
TAAGTCG40650.010.35039394
GGCGCCC73600.010.16837766-67
GGGAACA178800.010.0303222
TGAGGTT304700.09.9371668
GACGTGC39950.09.88369892-93
GTTTTTA161750.09.8204581
GCGCCCG73400.09.81632268-69
AGTTTTG299600.09.717623
AAGTTTT318400.09.6412652
TAGGGCG29000.09.6377338
Produced by FastQC (version 0.11.5)