FastQCFastQC Report
Tue 21 Jan 2020
Mp.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMp.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24590389
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG76850.022.50665994
GGGGGAT72000.015.2577791
CGGGAAC113800.014.643761
CGTGCAT47400.011.61052694
GGGGATA96800.011.4449382
GTTTTTT255900.011.0233651
TTGAGGT283800.010.7597777
CTTAAAC174500.010.5635911
GTTTTAT159700.010.5515311
TTAATAC60000.010.5511423
TTTAATA95500.010.1871982
CGCGAAC33250.010.0798181
TTTGAGG335050.010.0725696
GTTTTTA160700.010.0513751
GGGAACA156500.09.9641972
GAGGTTT306450.09.9037539
TTAAACG93900.09.8650512
AGTTTTG295200.09.8554293
TGAGGTT305800.09.7878068
GGGCCCT33850.09.7636358
Produced by FastQC (version 0.11.5)