FastQCFastQC Report
Tue 21 Jan 2020
PRO.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24715119
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64500.030.38784294
GGGGGAT62600.017.3293721
TAGGGCG32150.012.8887358
CGGGAAC139300.011.831871
CGTGCAT52850.011.81993194
GGGGATA84500.011.7912092
CTTAAAC157750.011.6581331
GTTTTAT164250.011.5652761
CGCGTAT18950.011.0561661
GTTTTTT286250.010.9138571
GTAGGGC35450.010.9009197
CTTTATT148400.010.4788541
TTAAACG87350.010.3937662
GGGTCTA64300.010.3544335
AGGGCGG41800.010.2473789
TAAACGT86500.010.1191253
TTGAGGT280700.010.0017547
TTTGAGG319900.09.9259346
CTTAGAT106950.09.6643671
GTTTGGG209250.09.6566291
Produced by FastQC (version 0.11.5)