FastQCFastQC Report
Tue 21 Jan 2020
PRO.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24715119
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT250240.10124976537640787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83050.022.4238194
GGGGGAT68900.014.7719371
CGGGAAC113750.014.2997761
CGCGTAT18750.013.1367261
TTTAATA99200.012.1339062
TAGGGCG28750.012.1237238
TTAATAC63250.011.9032913
CTTAAAC172100.011.7738411
TTAAACG93700.011.3085742
TAAACGT90350.011.1620553
TTGAGGT284150.010.7783027
ATTTAAT91700.010.7443241
AGTTTTG284800.010.4926113
GTTTAAT91450.010.46878051
GTTTTAT164050.010.3118771
TTTTGAG356600.010.0742035
GGGCCCT32550.09.9944668
GTAGGGC34450.09.98286157
TGAGGTT297400.09.9386818
GTTTTTT260450.09.8854851
Produced by FastQC (version 0.11.5)