FastQCFastQC Report
Tue 21 Jan 2020
PRO.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24641499
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT291740.11839377141788329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG70800.030.99656394
GGGGGAT70000.017.8848111
CGCGTAT22000.015.4429521
CGGGAAC148900.013.5651681
CGTGCAT50250.012.95281894
GTAGGGC36100.012.8920787
TAGGGCG37950.012.8767918
AGGGCGG40250.012.4878599
TTTGAGG346050.012.0099936
CTTAAAC151300.011.9657751
AGTTTTG289900.011.9280843
GCGTGCG32500.011.7425012
GGGGATA103100.011.6463862
TTGAGGT303700.011.631277
CTTTTAT174050.011.1771841
CTTAGAT109150.011.0008461
AAGTTTT330400.010.9589692
TGAGGTT315300.010.74576958
GTTTTAT150450.010.7341441
GAGGTTT320850.010.6759359
Produced by FastQC (version 0.11.5)