FastQCFastQC Report
Tue 21 Jan 2020
PRO.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24923645
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG68450.034.77055794
CGCGTAT21600.014.2268081
GGGGGAT62800.013.8643431
CGGGAAC145650.013.5541611
GTTTTAT148350.011.6126551
ATCGGGC30850.011.31946
GTTTTTT286500.010.6609651
GTTTTTA159050.010.5679521
GCGTGCG29550.010.5568562
CGTGCAT50100.010.48091494
CTTAAAC158400.010.2879811
CTTTTAT183900.010.20329951
CTCGAAT151200.010.19281
TCGGGCG25600.010.1851027
GGGGATA87650.09.9867292
TTAAACG84050.09.9159112
TTTGAGG320950.09.777776
TTGAGGT276050.09.7152067
GGGAACA187650.09.7016482
CTTAGAT102900.09.5926321
Produced by FastQC (version 0.11.5)