FastQCFastQC Report
Tue 21 Jan 2020
PRO.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePRO.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24923645
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95650.023.98824994
CGCGTAT21100.014.782731
CGGGAAC119650.014.5519511
GCGTGCG25050.012.2658752
TTAATAC61050.011.0571873
GTTTTTT253400.010.7108581
GTTTTAT159300.010.6669291
CTTAAAC163100.010.5896661
TTAAACG89850.010.5699852
TTGAGGT268750.010.272327
GTTTTTA156150.010.2262041
GGGGGAT73400.010.211561
TTTAATA100850.010.2018442
CTTTTAT170950.010.1578561
TAGGGCG33250.010.080998
GCGGCCC26900.010.0377886
AGTTTTG273700.09.9334563
GTAGGGC33900.09.8876967
TTTGAGG330550.09.7461086
ATTTAAT98150.09.7235741
Produced by FastQC (version 0.11.5)