FastQCFastQC Report
Tue 21 Jan 2020
Pa.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24902289
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT280090.11247560415028515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG60700.034.6099694
GGGGGAT66000.016.872241
TTAATAC62950.012.95272353
GGGGATA82500.012.7071282
CGTGCAT50100.012.57978894
TTGAGGT297850.012.2797887
TAGGGCG27450.012.2210628
CGGGAAC126200.011.7774191
TTTAATA104400.011.6481992
TTTGAGG338150.011.6430336
GTTTTAT166600.011.4104811
ATTTAAT93750.011.1325721
AGTTTTG310300.011.0813683
GAGGTTT320500.011.0049149
TGAGGTT312900.010.9446188
CTTAAAC158400.010.9128331
AAGTTTT327350.010.6892292
CTTTTAT177100.010.6813531
GTAGGGC32300.010.6745117
TTTTGAG386650.010.6163855
Produced by FastQC (version 0.11.5)