FastQCFastQC Report
Tue 21 Jan 2020
Pa.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24902289
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT298240.11976409076290137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG79150.025.32409394
TTAATAC65250.014.60923
CGGGAAC103400.014.2558071
GGGGGAT74400.013.8750041
TTTAATA105450.012.8321522
ATTTAAT93900.012.8258841
TTGAGGT292600.012.3163067
TTTGAGG348850.011.3034296
AGTTTTG315450.011.0408963
CTTAAAC166500.010.9477521
TGAGGTT322600.010.9115048
GAGGTTT324800.010.8805479
TAGGGCG26700.010.7977568
GTTTTTT257400.010.6404471
TTTTGAG379100.010.5486725
TTAAACG84550.010.4494412
AAGTTTT336750.010.4392012
CGCGAAC32950.010.3019761
CGTGCAT48500.010.2145694
CTTTTAT164250.010.1351621
Produced by FastQC (version 0.11.5)