FastQCFastQC Report
Tue 21 Jan 2020
Pa.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24724086
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT259800.10507971861932532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65150.034.0692694
GGGGGAT68100.015.1776341
CGGGAAC123850.012.931851
CGCGTAT18100.012.6042131
TAGGGCG30200.012.3333578
TTGAGGT296600.011.45908457
TTTGAGG319000.011.4279756
GGGGATA87500.011.386862
CGTGCAT52900.011.38412994
GTAGGGC32350.011.3697537
GTTTTTT286050.011.3608721
GTTTTAT155450.011.2015751
TTAATAC61800.011.1499133
TTTAATA102800.011.1414122
AGTTTTG300850.010.9257933
TTAAACG84900.010.5839522
TGAGGTT310600.010.41793258
TTTTGAG377150.010.2461855
AGGGCGG41050.010.2076929
AAGTTTT320000.010.1846542
Produced by FastQC (version 0.11.5)