FastQCFastQC Report
Tue 21 Jan 2020
Pa.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24724086
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT257260.10405238033875146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG81700.025.7361794
TTAATAC63600.014.2499363
CGGGAAC107300.013.9040321
GGGGGAT77000.013.8222971
TTTAATA100150.012.0658472
TTAAACG94600.011.8893922
ATTTAAT91400.011.6954551
TAAACGT93500.011.1345293
TTGAGGT284950.011.1074387
CTTAAAC176600.011.1059881
TAATACA91650.010.9535964
GTTTTTT247500.010.7412861
GGGGATA100200.010.6683082
GTTTTAT155500.010.5506641
CGTGCAT46850.010.39463494
TTTGAGG338050.010.3113566
AGTTTTG296050.010.235713
CTTTTAT160600.010.1287991
TAGGGCG28600.010.0753738
CTTTATT134650.010.0098391
Produced by FastQC (version 0.11.5)