FastQCFastQC Report
Tue 21 Jan 2020
Pa.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24714658
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT255900.10354179289068051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG60650.035.380494
CGGGAAC132700.012.6684071
GGGGGAT69700.012.2265531
TAGGGCG31800.011.2758518
CTTAAAC157250.011.1051991
TTTAATA94100.011.0852152
GTTTTAT161900.011.0451111
GTTTTTT283350.010.7154421
CTTAGAT107400.010.6663681
TTGAGGT295850.010.6562117
CTTTTAT170450.010.6003551
TTAATAC57850.010.4646753
TTTGAGG325900.010.3595146
AGTTTTG296050.010.1613913
GTTTTTA159950.010.1607771
AAGTTTT314800.010.0295322
CTTTAAT140000.09.9788151
GGGGATA85400.09.9788142
CGCGTAT18850.09.8817621
TTAAACG81900.09.8366662
Produced by FastQC (version 0.11.5)