FastQCFastQC Report
Tue 21 Jan 2020
Pa.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePa.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24714658
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT254790.10309266670815351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG76250.027.16287894
TTAATAC63250.014.5410243
TAGGGCG29050.013.1116718
TTTAATA100450.013.0403582
ATTTAAT88300.012.6778811
CGGGAAC107800.012.3666911
TTGAGGT283000.011.5880047
TTAAACG91300.011.1420142
GTAGGGC34100.010.8974777
GTTTTTT246100.010.7019221
GTTTTAT161150.010.5785491
AGTTTTG295450.010.4865393
CTTAAAC173650.010.4858011
GGGGGAT79300.010.4850421
CTTTTAT161000.010.41529751
CGTGCAT46600.010.27574694
TAAACGT87500.010.139483
TGAGGTT313850.010.1381518
AGGGCGG39600.010.0877339
GAGGTTT309150.010.0669039
Produced by FastQC (version 0.11.5)