FastQCFastQC Report
Tue 21 Jan 2020
RGA.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24973480
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG63550.038.66752694
CGGGAAC129700.013.9258911
TAGGGCG29550.013.0753058
GGGGGAT68000.012.3908441
CGTGCAT49850.011.49051694
GTTTTTT277850.010.9906831
GTTTTAT167500.010.7276291
TTTAATA97700.010.67295552
TTAATAC60000.010.6291993
GTAGGGC35300.010.5498557
CTTAGAT104500.010.1566331
CTTTTAT169900.010.0007071
ATTTAAT93800.09.8760081
TTGAGGT279150.09.8055277
CTTAAAC163150.09.7867441
AGGGCGG41000.09.7644069
CTTTATT147700.09.675851
GGGGATA83250.09.6177932
GGGTAGG41150.09.6156875
CAAGTCG87600.09.54670394
Produced by FastQC (version 0.11.5)