FastQCFastQC Report
Tue 21 Jan 2020
RGA.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24973480
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG82400.030.38463694
GGGGGAT74500.013.8195681
CGGGAAC113950.013.7367321
TTAATAC63750.012.2768673
TAGGGCG29300.011.2888378
CGCGTAT18050.011.0981271
TTTAATA108000.010.9564142
ATTTAAT90400.010.8220451
TTAAACG95450.010.6399362
GTTTTTT255750.010.4193081
TAAACGT92600.010.3636973
TTGAGGT273350.010.2767727
CTTAAAC176850.010.273491
CTTTTAT166500.010.1846381
CTTTAAT143950.010.1295731
CGTGCAT46100.09.940680594
GTTTTTA156650.09.8733871
GTTTTAT176400.09.7715081
GGGGATA99550.09.6869612
GTAGGGC32750.09.5306387
Produced by FastQC (version 0.11.5)