FastQCFastQC Report
Tue 21 Jan 2020
RGA.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24783546
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG58450.035.527594
CGGGAAC137300.013.2612231
CCCCGCG35050.012.56739594
TAGGGCG29350.012.5341918
GGGGGAT63450.012.329771
TTTAATA97650.011.731142
TTAATAC54850.011.6311123
GTTTTAT162200.011.5125321
GTTTTTT284700.011.2205461
CTTAAAC160700.011.0694191
GTAGGGC33950.010.6987297
GTTTAAT90500.010.5483041
CTCGAAT162500.010.5169551
TTGAGGT283050.010.4140077
TGCGCCA51050.010.2164419
CTTTAAT143650.010.1464961
TTAAACG86900.010.12792
ACTTGCG60800.010.0333296
GTTTTTA156300.010.0105431
TTTGAGG322800.09.9538916
Produced by FastQC (version 0.11.5)