FastQCFastQC Report
Tue 21 Jan 2020
RGA.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24783546
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG79000.025.02857894
CGGGAAC113550.015.2303411
TTAATAC59600.014.6644373
TTTAATA100550.013.3619422
ATTTAAT88250.012.2742521
TTAAACG88950.011.9163362
TAAACGT88600.011.4911693
CTTAAAC168200.010.9175431
GTTTTTT257350.010.6942751
CTTTTAT160550.010.3084541
CTTTAAT143750.010.219581
TAATACA90550.010.06286054
CTCGAAT146650.09.7955811
TTGAGGT273950.09.7577657
GTTTTAT166800.09.7270961
AAAAAAC354650.09.7134181
CCCCGCG50300.09.42968794
GTTTTAC187550.09.3945491
GGGAACA160350.09.3935532
GTTTTTA161500.09.3842351
Produced by FastQC (version 0.11.5)