FastQCFastQC Report
Tue 21 Jan 2020
RGA.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRGA.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24757151
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTTTGAGGTTTACACAAAAGCAAAGGGAAATTAACCGGTGAAGCTT252000.10178877206024231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG61700.040.08788794
GGGGGAT71700.015.3892011
CGGGAAC144600.014.6175451
TAGGGCG32050.013.21904858
GGGGATA89100.012.6451652
CGTGCAT51350.012.40519694
GTAGGGC35850.012.07760057
GTTTTAT165900.011.1131121
AGGGCGG40700.011.0959429
GTTTTTT277050.010.9230031
TTAATAC64000.010.8390983
TTGAGGT296900.010.2554447
TTTAATA102250.010.1993282
GTTTTTA164050.010.1883781
GGGAACA194550.010.0748292
TTTGAGG323150.010.0707046
GTTTGAT338900.010.05602551
GGGTAGG43100.010.0459845
CTTAAAC161500.09.9744841
ATTTAAT92900.09.8727321
Produced by FastQC (version 0.11.5)