FastQCFastQC Report
Tue 21 Jan 2020
Sm.1.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.1.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24706636
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG53550.036.08984894
GGGGGAT63100.017.1889321
CGTGCAT51600.013.15338494
CGGGAAC141700.012.6477481
GGGGATA85250.011.9037662
TAGGGCG31700.010.8666288
GTTTTTT281900.010.8160471
GTTTTAT153650.010.6945971
CTTAAAC164300.010.1430321
TTGAGGT287900.010.0570597
GGCGCCC65750.09.99085366-67
GTTTGAT334550.09.9487231
TTTGAGG318700.09.9176796
ACTCACG51550.09.8428346
TTAAACG88750.09.8083412
GTTTTTA164000.09.7358221
GTTCATT152900.09.7119291
CTTTTAT172450.09.6636711
GCGCCCG65250.09.49696468-69
CTTTAAT144300.09.4842571
Produced by FastQC (version 0.11.5)