FastQCFastQC Report
Tue 21 Jan 2020
Sm.1.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.1.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24706636
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG75150.025.37396894
CGGGAAC109200.014.97701551
GGGGGAT72500.014.4194861
TAGGGCG29550.013.8366348
GTAGGGC31200.013.4027277
CGCGTAT16950.011.5129031
TTAATAC59300.011.43940453
TTTAATA95500.011.1413382
GGGTAGG38400.010.8897155
TTGAGGT276950.010.7370227
GTTTTTT248800.010.6819581
GGGCCCT35700.010.6721258
CGTGCAT49600.010.56936994
CTTAAAC173250.010.5127931
GGGGATA96250.010.4269742
ATTTAAT82450.010.3125411
AGGGCGG41250.010.1373359
GTTTTTA156950.09.82838251
CGCAAAC101100.09.696981
CGCGAAC34500.09.6965821
Produced by FastQC (version 0.11.5)