FastQCFastQC Report
Tue 21 Jan 2020
Sm.2.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.2.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24879989
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG59000.038.3160794
GGGGGAT64150.015.3036331
CGGGAAC140600.013.6008241
TAGGGCG32550.013.2935428
CGTGCAT49800.013.1191494
GTAGGGC38150.011.2202377
CTTAAAC160350.011.0841691
GGGGATA83200.011.0167042
TTGAGGT284050.010.8599417
GTTTTAT160600.010.7482341
GTTTTTT285950.010.6901561
GTTTTTA165850.010.4641061
TTAATAC60550.010.2967323
TTTGAGG325500.010.2774816
TTTTGAG367150.010.1760855
ATTTAAT90150.010.0641611
CTTTTAT174350.010.0073261
TGAGGTT301000.09.8464968
AGGGCGG45400.09.8383929
TAAGTCG44700.09.70085394
Produced by FastQC (version 0.11.5)