FastQCFastQC Report
Tue 21 Jan 2020
Sm.2.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.2.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24879989
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG79300.028.70696894
CGGGAAC112150.014.9298221
TTAATAC62050.013.3422683
TAGGGCG30450.013.13598258
GTAGGGC34300.012.2039337
GGGGGAT67500.011.920481
CGTGCAT47000.011.74557794
TTTAATA101300.011.7079852
ATTTAAT93400.011.2541571
CTTAAAC176500.011.173071
GTTTTAT170350.011.0576881
GGGCCCT33700.010.9030658
GTTTTTT252200.010.5856671
TTGAGGT268950.010.4452027
GGGTAGG38450.010.2819155
CGCAAAC95500.09.9352361
TTTGAGG319450.09.8277096
GAGGTTT290050.09.7976019
TAAACGT92250.09.7810793
TTAAACG98500.09.7742972
Produced by FastQC (version 0.11.5)