FastQCFastQC Report
Tue 21 Jan 2020
Sm.3.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.3.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25179134
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64050.037.1705294
TAGGGCG33300.016.3695568
CGGGAAC135050.013.8684121
GTAGGGC40100.013.1289297
AGGGCGG46000.012.4578349
GGGGGAT61450.012.3583651
GGGTAGG46500.011.32193855
TAAGTCG47550.010.993628594
CGTGCAT55900.010.876163594
GTTTTTT287400.010.8289161
CGCGAAC32900.010.7624961
CCCCGCG33800.010.7588894
CTTAGAT108800.010.3200881
GTTTTAT169550.010.1946281
TTGAGGT288700.010.0055317
GTTTTTA165050.09.8233361
TTTGAGG325150.09.8152666
CTTTATT149000.09.7870861
TTTAATA101900.09.7844162
ATTTAAT96550.09.699271
Produced by FastQC (version 0.11.5)