FastQCFastQC Report
Tue 21 Jan 2020
Sm.3.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSm.3.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25179134
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG77450.028.38710494
CGGGAAC115350.014.7685561
TAAGTCG49650.012.97712694
TAGGGCG28550.012.2272758
CTTAAAC180100.012.0950221
CGCGTAT20900.012.0260231
TTTAATA102250.011.8354452
TTAAACG96250.011.6544252
TTAATAC62800.011.5621743
GTTTTTT262350.011.12400151
TAAACGT95600.010.9059793
GGGCCCT31350.010.6897988
CGTGCAT49700.010.23479394
ATTTAAT89400.010.0483411
TTGAGGT259500.010.0444297
CTTTAAT150850.09.9353941
GTTTTAT169700.09.9014751
GGGAACA162750.09.723722
GTAGGGC36600.09.5379447
CTTTTAT173350.09.3170881
Produced by FastQC (version 0.11.5)