FastQCFastQC Report
Thu 3 Feb 2022
S35_Galicia.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS35_Galicia.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25577451
Sequences flagged as poor quality0
Sequence length20-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGA6407502.5051362624055074No Hit
CTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGG4330471.6930811440123565No Hit
GTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGC3191311.2477044721931048No Hit
GCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCT2987321.1679506296385829No Hit
GTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCC2510450.9815090643708008No Hit
CGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTC1968290.7695411086898377No Hit
CCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCC1902800.7439365244019038No Hit
GTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGT1782720.6969889220000852No Hit
GTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGA1697900.6638268997172548No Hit
GTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATAC1692890.6618681431546873No Hit
CTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATT1488130.5818132541823655No Hit
GGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGA1245770.487057916756443No Hit
CTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCCT1243070.48600229944727485No Hit
CGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGAT1218430.47636881407768117No Hit
CCCATGTTCAACCGCCTTGCGGACACGAGCTCGTCGGCGATCGTGACCAC1111630.4346132849594747No Hit
CTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGATT1091160.4266101418784851No Hit
GGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATT1026600.4013691591081535No Hit
GTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGC1015120.3968808306973201No Hit
GGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTG1000170.3910358385595187No Hit
CGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACG981880.38388500871333897No Hit
GTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCG977340.38211000775644144No Hit
GTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAA968210.37854045737395803No Hit
CTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCCGC924940.36162321257110414No Hit
CCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCCATGTTCAAC918420.35907409225414993No Hit
GCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGC909560.3556101036025834No Hit
CTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCCA809310.31641542388254407No Hit
GATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTT800070.3128028668689464No Hit
CCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGTCCGAGG794530.31063689653828286No Hit
CACGGTCCGAGGGGGAGGCTCCGGACACCGCAGCAACTGCGGTGCTCTCC792880.30999179707156904No Hit
GCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGAT782210.30582015385348604No Hit
GTCGAACGAGAGCTCGACTCGGATTGTCGTCGCAGACTGGTTCGGGATAC778960.3045495033887466No Hit
CGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCCCC778220.30426018605215976No Hit
CCGGATTTTCACGGTCCGAGGGGGAGGCTCCGGACACCGCAGCAACTGCG775690.30327103353653184No Hit
CGCAGGTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTC765320.29921668113057864No Hit
ATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCA759930.29710935620598006No Hit
GAACCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACT759160.29680830978818024No Hit
AGACATTTGAAAGATGCATCGCCAGCACTGAGACCGTGCGATCAGCACAA728010.28462961379537No Hit
GTCCGCCAATCGGCATTGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGC701460.2742493769218833No Hit
GGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACC674590.2637440298487914No Hit
CGCCAATCGGCATTGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACC666210.26046770649663253No Hit
CTCGGGACTCCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACAC628550.24574379988060577No Hit
GGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACG616890.2411850969824945No Hit
CTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCCTTAGAG615640.240696385265287No Hit
GCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGC609620.23834274963521582No Hit
GCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACG604940.23651301296599103No Hit
GGTAACTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCT589870.2306211045033377No Hit
GTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGA585170.22878354844663762No Hit
CCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTA583580.2281619071423497No Hit
CTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCG557520.2179732452620083No Hit
CAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCG553070.21623343154874974No Hit
ACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTC532050.20801525531218887No Hit
GCCCTCTCCCGGATTTTCACGGTCCGAGGGGGAGGCTCCGGACACCGCAG516200.2018183907379981No Hit
GCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGC513800.20088006424095975No Hit
GTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCC512090.2002115066118199No Hit
CAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCCGCCT502270.196372187361438No Hit
GGCAGACATTTGAAAGATGCATCGCCAGCACTGAGACCGTGCGATCAGCA501320.19600076645636036No Hit
CTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAA493520.1929512053409857No Hit
CTCGGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCG476320.18622653211221085No Hit
CGGGACTCCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCA469890.1837125990388956No Hit
CCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGA461940.18060439251745608No Hit
CCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCG460230.17993583488831627No Hit
CCATGTTCAACCGCCTTGCGGACACGAGCTCGTCGGCGATCGTGACCACG456750.17857526146761066No Hit
GTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGATTCCC444720.17387189990120594No Hit
CGGCATTGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACA443000.17319943257832845No Hit
GCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCC442500.1730039478914455No Hit
CTAGCGTGTGTGCTCTGGGCGCACCTCTCGTCTCGCGAACGAGGCGAACG431560.1687267429424457No Hit
GCTTCATCGAGTAAGTAAGGAAACGATGAGAGTAGTGGTATTTCACCGGC424590.1660016864072968No Hit
GGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAG423190.16545432928402443No Hit
CCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGAT422160.16505163082904548No Hit
CAGACATTTGAAAGATGCATCGCCAGCACTGAGACCGTGCGATCAGCACA421070.16462547421164056No Hit
GCAGGTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCG420050.16422668545039926No Hit
CCCGAAACGTGTCCGCCAATCGGCATTGGGCTTGTAGCGGGGGCCGATGA418700.16369887679581518No Hit
GTTCAACCGCCTTGCGGACACGAGCTCGTCGGCGATCGTGACCACGAACG418650.1636793283271269No Hit
TGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTA416840.16297167376061047No Hit
CTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGT390290.15259143688712373No Hit
TGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACT388550.15191115017677095No Hit
GTGCTCTCCGCGTTCGGCTCCCTATCTCCCTGCTAGAGGATTCCAGGCAG381350.1490961706856559No Hit
CGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGA378920.14814611510740458No Hit
GTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGGGACTCCTGGTATTG373670.14609352589513316No Hit
CTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTC369740.14455701625623288No Hit
GCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGGGACTCCTGGT368010.14388063923961775No Hit
AACCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTG364330.14244187194415894No Hit
AGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGG351220.13731626345408696No Hit
AGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG350560.13705822366740142No Hit
CGGATTTTCACGGTCCGAGGGGGAGGCTCCGGACACCGCAGCAACTGCGG345570.1351072864923092No Hit
GTTCAACTACCGCCGGGACTCCGGCCAGCGAGTTCGGGGTTCGGCGACAT333660.13045084125075637No Hit
ATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAAC332780.1301067882018423No Hit
CAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTAC331760.12970799944060102No Hit
AGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAAC329530.1288361377371029No Hit
CGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGG326570.1276788683907556No Hit
CAGGTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGG326260.12755766788488815No Hit
GGCAGAAATCACATCGCGTCAGGCACCCTTTGGGGCCATCGCGATGCTTT321160.12556372407868166No Hit
GGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG320710.12538778786048696No Hit
TGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGG317410.12409758892705923No Hit
GTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGGGAC314710.12304197161789109No Hit
TGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCAC313460.12255325990068362No Hit
CGGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGAC306970.12001586866494242No Hit
CCCGGATTTTCACGGTCCGAGGGGGAGGCTCCGGACACCGCAGCAACTGC304880.11919874267377151No Hit
CGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGA300590.11752148406031547No Hit
ACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCCTTAGA296940.11609444584606964No Hit
CGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG296890.11607489737738136No Hit
TGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG295930.11569956677856602No Hit
AACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCCGCCTG293550.11476905966900298No Hit
CCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCT293210.11463613008192254No Hit
GTCGGGTTCCGAAAACCACTTAACCTCGAACTGGAACCGACGTCCTATTC289090.11302533626200671No Hit
GCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGTCCGAGGGGGAGG284630.11128161285501045No Hit
CTCAGCTTCTCCGGGTCTTTTCGGGTTACCCCGACGAGGCCTTCGCGACA283070.11067170063193553No Hit
ACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCC280930.10983502617207634No Hit
GGTCGAACGAGAGCTCGACTCGGATTGTCGTCGCAGACTGGTTCGGGATA280370.10961608332276739No Hit
CGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATA280180.10954179914175184No Hit
GTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCCCCCGTCACCCG273210.10681674260660298No Hit
GTTTGACGCCCGCGAAGGGCGTCCACCCATGTTCAACCGCCTTGCGGACA270320.10568684111641931No Hit
AGAAAAGATAACTCTCCCCGGAGCTCCCCTCAGCTTCTCCGGGTCTTTTC267900.10474069523190564No Hit
GTGGTAACTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGT263920.10318463712431704No Hit
GTCTGATTGTATTGACGAGGCTACGCACCAGCATTCACTCTCAGGCGCTA262590.1026646478572083No Hit
GCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATC258640.10112031883083267No Hit
CGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGTCCGAGGGGGAG256430.10025627651480985No Hit
GTGCTCTGGGCGCACCTCTCGTCTCGCGAACGAGGCGAACGAGGCACCCC255780.10000214642186198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)