FastQCFastQC Report
Thu 3 Feb 2022
S35_Galicia.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS35_Galicia.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25577451
Sequences flagged as poor quality0
Sequence length20-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCG6263532.448848401664419No Hit
GGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTT2000360.7820794965065128No Hit
GTTCCGACCGCCTCGTGCGGGAGGAACGCTTTTATTAGATCAAAACCCAT1678610.6562851004973091No Hit
TTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCG1648450.6444934641845272No Hit
CGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGAGAGTTATCTTTTC1606590.6281274861986833No Hit
TTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGC1510320.5904888645862326No Hit
GCAGGTTCGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGA1406120.5497498558398176No Hit
GGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTT1252030.4895053850362181No Hit
GCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGTTCGCGGAT1215200.47510598300041706No Hit
GGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTC1199700.4690459577070444No Hit
GTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGGA1149680.44948966963127013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1124450.4396255123311545No Hit
GGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCA1111390.43451945230977085No Hit
GTTTTACCCTACTGATGACTGCGGCAACCGTCGTTGCGTTAGTAATCCTG1110310.4340972053861036No Hit
GTTAGTTTTACCCTACTGATGACTGCGGCAACCGTCGTTGCGTTAGTAAT1076560.4209019890215018No Hit
GCCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCG995690.38928429576504714No Hit
CGAAGGCCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAG995040.38903016567209925No Hit
GGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTGGCC989600.3869032922788123No Hit
CGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTC982920.38429161686205554No Hit
AGTCGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCCA962640.3763627579620815No Hit
CGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCT932750.3646766833802164No Hit
GTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTGGCCC918430.3590780019478876No Hit
GTTCGTGGTCACGATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACA904860.3537725475458833No Hit
CATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCC861520.3368279348868658No Hit
CGCAGGTTCGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCG850030.3323356967822947No Hit
GCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGTTCGCGGATGCACGTT837430.32740948267284337No Hit
GGCGAGTTCGCGGATGCACGTTCGTGGTCACGATCGCCGACGAGCTCGTG813330.3179871207650833No Hit
GTTTCGGGCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTCACGATCGCC807860.31584851829058336No Hit
GTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGG805420.31489455301859437No Hit
CGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACC795090.3108558393875918No Hit
TGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGG793980.3104218633827116No Hit
GACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCAT792990.31003480370268327No Hit
CTCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGACGAAGCAGGCGGG786630.30754823848553164No Hit
CGATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACATGGGTGGACGC773190.30229361010211686No Hit
CGGGAATCAGTCGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTA718820.28103660525046065No Hit
GTTCGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCT672620.26297382018247245No Hit
CAGTCGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCC654740.2559832877795367No Hit
GTCGAGACGAAGCAGGCGGGAATCAGTCGATGTGCTCGGTGCAAACCGCG654140.2557487061552772No Hit
CCGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTT653310.2554242015750514No Hit
CACGTTTCGGGCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTCACGATC647280.2530666562512425No Hit
CCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTGGCCCTC644770.25208532312308995No Hit
CGACAATACCAGGAGTCCCGAGCGCGCGTGGCCCTCGCGGTCACGTGACG636180.2487268962024402No Hit
GTCGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCCAA619880.24235409541005473No Hit
GGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGG615850.24077848883377784No Hit
CGGACACGTTTCGGGCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTCAC611500.23907777205789582No Hit
CAGGTTCGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGAC604530.23635271552274695No Hit
GCTGAGGGGAGCTCCGGGGAGAGTTATCTTTTCTGTATCAGCGTTGTAGC603390.23590701043665374No Hit
GTCACGATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACATGGGTGG593140.23189957435555247No Hit
GTCGGCGGTCGGTATTTCCCGCGTCGGCGAGGTTTGACGCTCTCTCGGGC583230.22802506786153165No Hit
CATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGC572580.22386124403092397No Hit
AGCGTCTCGGCCTCGTCGTCGAGACGAAGCAGGCGGGAATCAGTCGATGT552100.21585419125619673No Hit
CCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGAGAGTTATCTTTT551810.21574081013780458No Hit
CCTCGCGGTCACGTGACGAACAGGCTCTAAACCTGCGAGCGTCTCGGCCT548410.21441151426700022No Hit
CCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGAGAGTTATCTTT542090.21194058782479924No Hit
CTCGGCCTCGTCGTCGAGACGAAGCAGGCGGGAATCAGTCGATGTGCTCG533430.2085547930479859No Hit
AATCAGTCGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAG526110.20569289723201892No Hit
CATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTGGCCCTCGTT524100.2049070487907493No Hit
GCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATT518760.202819272334839No Hit
GTGACGAACAGGCTCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGAC514910.20131404024583996No Hit
GGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCT502530.19647383939861715No Hit
CGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGGATT500370.19562934555128267No Hit
CGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGC497290.19442515988008344No Hit
GCCTGAACGCCTCTAAGGCCGTATCCGTTCTAATCGTAGTGGTGTACAGA491520.19216926659345374No Hit
GGACACGTTTCGGGCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTCACG479420.18743853717088538No Hit
GTCTCGGCCTCGTCGTCGAGACGAAGCAGGCGGGAATCAGTCGATGTGCT463260.18112047209082718No Hit
CGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGA462320.18075296087948717No Hit
ACCGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGT450180.1760065926819682No Hit
GAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGAGAGTTATCTTTTCT445780.1742863274373979No Hit
GGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGAGAGTTA443630.17344574328380102No Hit
CTGATGACTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTACGAG437100.1708927132731092No Hit
GTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGGGAG435330.17020069748154343No Hit
CGAGACGAAGCAGGCGGGAATCAGTCGATGTGCTCGGTGCAAACCGCGTC433180.16936011332794657No Hit
CGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTT430930.1684804322369731No Hit
CTTTGCGATCTGTGTCGCGAAGGCCTCGTCGGGGTAACCCGAAAAGACCC430560.1683357735686797No Hit
GCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGTT427040.16695956137302345No Hit
GGACGCGGAACCGCTCTCATGTTTCGTGGCCCTCGTTTCCGATCTTTGCG425910.1665177659806679No Hit
CGCGAAGGCCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGG413980.16185350135163978No Hit
CCTGAACGCCTCTAAGGCCGTATCCGTTCTAATCGTAGTGGTGTACAGAC413640.16172057176455934No Hit
GTCGCGAAGGCCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGG409960.16028180446910054No Hit
CTCAGGATAGCTGGCGCTCGGAGGTGTGTCGAGAGATGAACTCACTGAGT405500.15853808106210426No Hit
GACTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTACGAGAGGAA403050.15758020609637763No Hit
CGAACAGGCTCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGACGAAG401130.15682954489874695No Hit
GACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCG398390.15575828881462817No Hit
AGGCTCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGACGAAGCAGGC397990.15560190106512178No Hit
GTTTCCGATCTTTGCGATCTGTGTCGCGAAGGCCTCGTCGGGGTAACCCG395240.15452673528726532No Hit
GGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTG389290.15220046751335778No Hit
GGTTCGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGC384140.15018697523846297No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGG382830.14967480535882954No Hit
CCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAG382190.1494245849596193No Hit
TTCTAATCGTAGTGGTGTACAGACTGAATTGAGTGTAGGCGACGACAATA381550.14917436456040908No Hit
CTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTACGAGAGGAACC376930.14736808605361026No Hit
GGTCACGATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACATGGGTG373090.1458667636583489No Hit
ATACGCTCTCCTGTGTGGGGCGTGCCGAGTCGGCGGTCGGTATTTCCCGC372250.14553834938438548No Hit
CCCGAGCGCGCGTGGCCCTCGCGGTCACGTGACGAACAGGCTCTAAACCT371750.14534286469750252No Hit
CAGGAGTCCCGAGCGCGCGTGGCCCTCGCGGTCACGTGACGAACAGGCTC368370.14402138821417348No Hit
GTTCGCGGATGCACGTTCGTGGTCACGATCGCCGACGAGCTCGTGTCCGC367440.14365778669657114No Hit
GCCTCGTCGTCGAGACGAAGCAGGCGGGAATCAGTCGATGTGCTCGGTGC361120.14118686025437016No Hit
CTCGCGGTCACGTGACGAACAGGCTCTAAACCTGCGAGCGTCTCGGCCTC360740.14103829189233907No Hit
CGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGT359440.1405300317064433No Hit
CTACTGATGACTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTAC355110.1388371343180366No Hit
GTCCCGAGCGCGCGTGGCCCTCGCGGTCACGTGACGAACAGGCTCTAAAC354960.13877848891197173No Hit
GCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTCACGATCGCCGACGAGC354270.13850872004407322No Hit
GCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTC351120.13727716651671035No Hit
GTTAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGGACATTTGGT345840.135212848223226No Hit
TCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGACGAAGCAGGCGGGA345640.1351346543484728No Hit
GTTCTAATCGTAGTGGTGTACAGACTGAATTGAGTGTAGGCGACGACAAT343690.13437226406962915No Hit
GCACGTTCGTGGTCACGATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTG341440.1334925829786557No Hit
AGCTCGTGTCCGCAAGGCGGTTGAACATGGGTGGACGCCCTTCGCGGGCG340570.1331524396234793No Hit
GACGAAGCAGGCGGGAATCAGTCGATGTGCTCGGTGCAAACCGCGTCATC336430.13153382641608813No Hit
GGAGTCCCGAGCGCGCGTGGCCCTCGCGGTCACGTGACGAACAGGCTCTA336170.13143217437890897No Hit
CACGATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACATGGGTGGAC334470.1307675264435068No Hit
CGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTG329840.12895733824297034No Hit
CTCTAAGGCCGTATCCGTTCTAATCGTAGTGGTGTACAGACTGAATTGAG326730.12774142349055814No Hit
GCCGAGTCGGCGGTCGGTATTTCCCGCGTCGGCGAGGTTTGACGCTCTCT325720.12734654442305451No Hit
ACTGAATTGAGTGTAGGCGACGACAATACCAGGAGTCCCGAGCGCGCGTG322750.12618536538296954No Hit
TCTAATCGTAGTGGTGTACAGACTGAATTGAGTGTAGGCGACGACAATAC322490.12608371334579038No Hit
CTCGTTTCCGATCTTTGCGATCTGTGTCGCGAAGGCCTCGTCGGGGTAAC319350.12485606951216521No Hit
AGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGGATTAC318650.12458239095052903No Hit
GCTCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGACGAAGCAGGCGG318400.12448464860708756No Hit
CCGAATCCCCGGAGCCGAGTGCTTCGTCGAGGAGTTGTGGTGTTCGAGTG317470.1241210470894852No Hit
ACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGGATTACGCC315610.12339384405428047No Hit
CTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGTTC312840.1223108588889487No Hit
CAGGCTCTAAACCTGCGAGCGTCTCGGCCTCGTCGTCGAGACGAAGCAGG312420.122146651751967No Hit
GTAGAATAAGTGGGAGACGGGGTGGCGGTTCACCGTCGTTCGACTCGTTC310490.12139208086059865No Hit
GGCGTGCCGAGTCGGCGGTCGGTATTTCCCGCGTCGGCGAGGTTTGACGC306940.12000413958372944No Hit
TCAGTCGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCC306360.11977737734694517No Hit
ATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCT305990.11963271867865176No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCGATCTGTGTCGCGAAGGCCTC301500.11787726619044252No Hit
CCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGTTCGCGGATGC300840.11761922640375698No Hit
GCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTGG300770.11759185854759335No Hit
GTTATCTTTTCTGTATCAGCGTTGTAGCTGCCTGGAATCCTCTAGCAGGG300330.11741983202313631No Hit
CTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGCTCCGGG300020.11729863151726887No Hit
TCGGACATTTGGTCGAGCACTCGCTCGAGCGGGCGGTGGTGCGACGCTAC295600.11557054688522324No Hit
AACCGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACG293270.1146595882443485No Hit
ATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACATGGGTGGACGCCC290410.11354141583537782No Hit
GCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTACGAGAGGAACCGC289160.11305270411817034No Hit
GAAGGCCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTGAGGGGAGC289000.11299014901836778No Hit
GGCGGACACGTTTCGGGCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTC287310.11232941077670329No Hit
CCGAGCGCGCGTGGCCCTCGCGGTCACGTGACGAACAGGCTCTAAACCTG287270.11231377200175265No Hit
CTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGGATTACGCCT286660.11207528068375538No Hit
CGGGAGGGGAGTGCTCGGATGTCGCCGAACCCCGAACTCGCTGGCCGGAG286410.1119775383403139No Hit
ACTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTACGAGAGGAAC285140.11148100723563109No Hit
CTTTTCTGTATCAGCGTTGTAGCTGCCTGGAATCCTCTAGCAGGGAGATA282530.11046057717010191No Hit
CGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCGTGGC280230.10956134761044015No Hit
CCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGAGTTCGCGGATG278940.10905699711828204No Hit
CTCGTGTCCGCAAGGCGGTTGAACATGGGTGGACGCCCTTCGCGGGCGTC278400.10884587365644842No Hit
CGTCGTTGCGTTAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGG277580.1085252787699603No Hit
GCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGG273650.10698876913106002No Hit
CGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCCAATGCCGATTGGC273390.10688711709388085No Hit
CGGTATTTCCCGCGTCGGCGAGGTTTGACGCTCTCTCGGGCGTCTTCTTC273240.10682847168781595No Hit
GATACGCTCTCCTGTGTGGGGCGTGCCGAGTCGGCGGTCGGTATTTCCCG271370.1060973589588736No Hit
CCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTCTGGCGA263940.10319245651179236No Hit
GATCGCCGACGAGCTCGTGTCCGCAAGGCGGTTGAACATGGGTGGACGCC262360.10257472490124211No Hit
CGGGCTCTGGCGAGTTCGCGGATGCACGTTCGTGGTCACGATCGCCGACG261970.10242224684547338No Hit
CGATGTGCTCGGTGCAAACCGCGTCATCGGCCCCCGCTACAAGCCCAATG258480.10105776373103012No Hit
GCACTCGCTCGAGCGGGCGGTGGTGCGACGCTACCATCCGCGGGATTACG257080.10051040660775774No Hit
TTTACCCTACTGATGACTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCT256360.10022890865864624No Hit
CCCGAACTCGCTGGCCGGAGTCCCGGCGGTAGTTGAACGCACGCGGTTCG255790.10000605611559964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)