FastQCFastQC Report
Fri 4 Feb 2022
S38_SantoTomePrincipe.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35449329
Sequences flagged as poor quality0
Sequence length20-151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGA15498274.37195017146869No Hit
GCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCT5785961.63217757944022No Hit
GTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGC5601511.5801455649555454No Hit
GTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCC4918161.3873774592461257No Hit
CTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGG4346771.2261924619221989No Hit
GTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGA2293140.6468782526179833No Hit
GTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATAC2180330.6150553653638974No Hit
GGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGA2121160.5983639351819607No Hit
GTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGT2078830.5864229475260307No Hit
CGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTC2073230.5848432279211829No Hit
GTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCG1919450.5414629992009158No Hit
GTCGAACGAGAGCTCGACTCGGATTGTCGTCGCAGACTGGTTCGGGATAC1863270.5256150264508532No Hit
CCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCC1797910.5071774419199867No Hit
GCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGC1768400.49885288378801196No Hit
GGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTG1753290.4945904617827886No Hit
GTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAA1711990.482940029697036No Hit
GTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGC1638340.46216389596542157No Hit
CGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGAT1623830.4580707296321462No Hit
CCCATGTTCAACCGCCTTGCGGACACGAGCTCGTCGGCGATCGTGACCAC1515930.4276329179601679No Hit
GGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATT1456020.41073273911616215No Hit
GTCCGCCAATCGGCATTGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGC1401160.39525712884438513No Hit
GAACCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACT1395530.39366894645593997No Hit
GGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACG1387720.3914658017927505No Hit
GCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGAT1342270.37864468464269097No Hit
CCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCCATGTTCAAC1341850.37852620567232736No Hit
GCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGC1302260.36735815225162655No Hit
CGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACG1217240.3433746235365978No Hit
GCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACG1144710.3229144337259529No Hit
GGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACC1140170.32163373247487986No Hit
CTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGATT1121940.31649118097552703No Hit
GGTAACTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCT1094130.3086461805807382No Hit
CTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCCT1027980.2899857427484735No Hit
GATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTT1004310.2833086064901257No Hit
CGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCCCC990150.27931417263215336No Hit
CTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCCGC961130.2711278399656027No Hit
GCAGGTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCG951920.26852976540120127No Hit
GGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAG939460.26501488928041483No Hit
CTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCCA930180.26239706822095277No Hit
GTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCC909410.25653800104368685No Hit
CTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATT894400.2523037883171216No Hit
GTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGATTCCC870830.2456548613374318No Hit
ACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTC865580.244173874207887No Hit
CCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTA839090.2367012362913837No Hit
CCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGTCCGAGG819680.23122581530386652No Hit
GCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCC809880.2284613059953829No Hit
ATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCA770920.2174709710302274No Hit
GCCCTCTCCCGGATTTTCACGGTCCGAGGGGGAGGCTCCGGACACCGCAG768920.21690678545706749No Hit
CGCAGGTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTC741510.2091746221769106No Hit
CAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCCGCCT739160.20851170412844766No Hit
CAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCG719990.20310398540970975No Hit
GTTCAACCGCCTTGCGGACACGAGCTCGTCGGCGATCGTGACCACGAACG719160.20286984839684835No Hit
ACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCC718780.20276265313794797No Hit
GTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTAGA715510.2018402097258315No Hit
CGCCAATCGGCATTGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACC712930.20111241033645516No Hit
GGTCGAACGAGAGCTCGACTCGGATTGTCGTCGCAGACTGGTTCGGGATA703170.19835918473943473No Hit
AGACATTTGAAAGATGCATCGCCAGCACTGAGACCGTGCGATCAGCACAA693570.19565109398826702No Hit
GTTCAACTACCGCCGGGACTCCGGCCAGCGAGTTCGGGGTTCGGCGACAT660480.18631664368033596No Hit
GCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGGGACTCCTGGT655330.18486386582944914No Hit
CGGCATTGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACA654200.18454510098061375No Hit
CTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCG652260.18399784097464864No Hit
GCCTACACTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGC646330.18232503075022943No Hit
CCATGTTCAACCGCCTTGCGGACACGAGCTCGTCGGCGATCGTGACCACG638240.1800429001067975No Hit
CACGGTCCGAGGGGGAGGCTCCGGACACCGCAGCAACTGCGGTGCTCTCC619470.17474801850269156No Hit
GCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGTCCGAGGGGGAGG608390.1716224304273855No Hit
CCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCG594000.16756311522849981No Hit
CCGGATTTTCACGGTCCGAGGGGGAGGCTCCGGACACCGCAGCAACTGCG581300.16398053683893424No Hit
CTCGGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCG564550.1592554826637198No Hit
CTAGCGTGTGTGCTCTGGGCGCACCTCTCGTCTCGCGAACGAGGCGAACG562450.1586630878119019No Hit
CGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGA560320.15806223017648655No Hit
GCGGGAAATACCGACCGCCGACTCGGCACGCCCCACACAGGAGAGCGTAT560220.15803402089782856No Hit
AGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAAC556540.15699591944321428No Hit
GTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCCCCCGTCACCCG548860.15482944684228014No Hit
CGGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGAC546250.15409318466930644No Hit
AACCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTG544640.15363901528291268No Hit
CGGGACTCCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCA539190.15210160959605187No Hit
GGCAGACATTTGAAAGATGCATCGCCAGCACTGAGACCGTGCGATCAGCA535120.1509534919546714No Hit
GCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATC527790.14888575182904026No Hit
GCACCGAGCACATCGACTGATTCCCGCCTGCTTCGTCTCGACGACGAGGC523810.147763022538452No Hit
TGGGCTTGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACT508050.14331724022195175No Hit
GTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGGGACTCCTGGTATTG505590.14262329196696502No Hit
GGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG500620.14122129081766258No Hit
CCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGA482480.13610412766910202No Hit
CTCAATTCAGTCTGTACACCACTACGATTAGAACGGATACGGCCTTAGAG481680.13587845343983804No Hit
CCCGAAACGTGTCCGCCAATCGGCATTGGGCTTGTAGCGGGGGCCGATGA481190.13574022797441385No Hit
CTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAA469210.13236075639118586No Hit
CCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGAT469080.13232408432893045No Hit
GTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGGGAC468750.13223099370935906No Hit
GCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACA468640.13219996350283528No Hit
AGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGG461000.13004477461336433No Hit
GCGTATCCGAACCGCGTGCGTTCAACTACCGCCGGGACTCCGGCCAGCGA458030.12920695903722185No Hit
GTGGTAACTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGT456820.12886562676546007No Hit
ACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACGGTCC456460.1287640733622913No Hit
AACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCCCGCCTG455910.1286089223296723No Hit
CTCGGGACTCCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACAC455270.12842838294626113No Hit
CTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGAGTC450210.1270009934461665No Hit
GTTTGACGCCCGCGAAGGGCGTCCACCCATGTTCAACCGCCTTGCGGACA447740.12630422426331397No Hit
CCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCT441130.1244395909440204No Hit
GTGCTCTGGGCGCACCTCTCGTCTCGCGAACGAGGCGAACGAGGCACCCC439470.12397131691829766No Hit
GCTCGGGACTCCTGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACA436320.12308272464057078No Hit
GGCAGAAATCACATCGCGTCAGGCACCCTTTGGGGCCATCGCGATGCTTT434130.12246494143796066No Hit
ACCGCCAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGC433390.12225619277589146No Hit
GGTCTCAACAGATCGCAGCGTGGTAACTGCTCTACCGACTCACAGCGCCC432220.12192614421559292No Hit
GCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCCCCC428990.12101498451493962No Hit
GGAGAGCGTATCCGAACCGCGTGCGTTCAACTACCGCCGGGACTCCGGCC424920.11986686687355916No Hit
GTACGTAAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTG423500.1194662951166156No Hit
TGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCAC421930.11902340944168505No Hit
GTCGGGTTCCGAAAACCACTTAACCTCGAACTGGAACCGACGTCCTATTC421640.11894160253357686No Hit
TGGTATTGTCGTCGCCTACACTCAATTCAGTCTGTACACCACTACGATTA421520.11890775139918727No Hit
AAGTCGTCTGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCC421240.11882876541894488No Hit
TCGCAGACTGGTTCGGGATACGGCGCCATCGGACGCCGACGAGCCGGCCC409070.1153956962062667No Hit
GTGCTCTCCGCGTTCGGCTCCCTATCTCCCTGCTAGAGGATTCCAGGCAG408870.1153392776489507No Hit
CAAAGCTGAGGGCTAGGTCTCAACAGATCGCAGCGTGGTAACTGCTCTAC406690.11472431537420638No Hit
GGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCAC406590.1146961060955484No Hit
TGCAAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGG399750.1127665914353414No Hit
GAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGATTTTCACG395450.11155359245304755No Hit
GCGGGTATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGGA394070.1111643044075672No Hit
GCTTCATCGAGTAAGTAAGGAAACGATGAGAGTAGTGGTATTTCACCGGC392300.11066500017532067No Hit
AGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG389480.10986949851716515No Hit
CGAACGAGAGCTCGACTCGGATTGTCGTCGCAGACTGGTTCGGGATACGG388710.1096522870714986No Hit
CGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCA387000.10916990840644684No Hit
CAGACATTTGAAAGATGCATCGCCAGCACTGAGACCGTGCGATCAGCACA383510.10818540458128276No Hit
CGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTGCAAACGGGCTCGA376610.10623896435388101No Hit
ATCCGAACCGCGTGCGTTCAACTACCGCCGGGACTCCGGCCAGCGAGTTC376420.10618536672443082No Hit
CAGGTTTAGAGCCTGTTCGTCACGTGACCGCGAGGGCCACGCGCGCTCGG374950.10577069032815826No Hit
GTAACTGCTCTACCGACTCACAGCGCCCCGCCTGGTACGTAAGTCGTCTG374250.10557322537755227No Hit
GTCTGATTGTATTGACGAGGCTACGCACCAGCATTCACTCTCAGGCGCTA374070.10552244867596788No Hit
TGTAGCGGGGGCCGATGACGCGGTTTGCACCGAGCACATCGACTGATTCC373020.10522625125005892No Hit
TGCGGATATGAGTACGACCTGGCGCGAGACTTCGCGTGGCCCTCTCCCGG363230.10246456286944106No Hit
AAACGGGCTCGAGTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGT363100.10242789080718566No Hit
CTCGACTCGGATTGTCGTCGCAGACTGGTTCGGGATACGGCGCCATCGGA358790.10121207089702601No Hit
GTCCCAACATCGAACTGTTTGACGCCCGCGAAGGGCGTCCACCCATGTTC355880.1003911808880783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)