FastQCFastQC Report
Tue 20 Oct 2020
S33.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052341
Sequences flagged as poor quality0
Sequence length15-30
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACCTCGGGCTGTACGGGACTACCCGG2144897.027032693922468No Hit
CTCCGGGCGGGACCTCGAATCGTC1018183.3357347688217014No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG295870.9693215797317535No Hit
GGGGCTGATTTTGGATTCGACGAAATTT294220.9639158927524809No Hit
AACGACCTCGGGCTGTACGGGACTACCCGG231770.7593188310218288No Hit
GCCTTCACGGGCGTCGCGTCGCGGTCCG198310.6496980514300336No Hit
GGGGACAGAACTAGGAAGCAACTTAAAAGC165480.5421412614121424No Hit
CTACGAGCCGGGCCTGTGCGAGGTGAAGTT151080.49496435686576307No Hit
CGCCGCGCTTCGGACCCCAGCGTG148170.4854306907386822No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC143460.46999991154330395No Hit
GTGCCTTCACGGGCGTCGCGTCGCGGTCCG124570.4081129860654494No Hit
CTCCGGGCGGGACCTCGAATCGTCC113120.3706007946032242No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC111080.36391739979248716No Hit
CGAGCCGGGCCTGTGCGAGGTGAAGTT108690.3560873441073589No Hit
GGTCGGTATTTCCCGCGTCGGCGA85410.27981801509071236No Hit
CTTTAACACTGAGGCGTGATGACGAGTC80420.2634699071958212No Hit
AACACTGAGGCGTGATGACGAGTC75370.24692522886532012No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC71470.2341481505506757No Hit
ACGAGCCGGGCCTGTGCGAGGTGAAGTT70790.23192035228043No Hit
AAGGAACTCCTCATACCCGTTGG69910.22903731922481793No Hit
GCCTCCGGGCGGGACCTCGAATCGTC65790.21553948264627054No Hit
CGCCGGTCGTGGCTGGGAACC60290.19752052604869508No Hit
CACCCTCCGACACACGTTCCTGACCCTT59420.19467025473235133No Hit
CGAGGCCAGGTGCGTCCTGTCGCGGTGCTG56930.1865125816545399No Hit
GTCGTCCGTCGGCCCCTGGC56060.18366231033819616No Hit
CTCGGTAGTATAGTGGTCAGTATCCCCGCC54450.17838767031599678No Hit
GTCGCCGCGCTTCGGACCCCAGC54170.17747034161648387No Hit
TCTTTAACACTGAGGCGTGATGACGAGTC49660.162694797206472No Hit
CACCCTCCGACACACGTTCCTGACCCTTT49410.16187575372476404No Hit
CGGAATTCTTGAACCAAGCGCAAGCGG48460.15876338849427374No Hit
GTCCGTCGGCCCCTGGC46760.1531938928186595No Hit
GAGGTGTAGAATAAGTGGGAGACGGG45990.15067123889499895No Hit
CTCGTAGTATAGTGGTCAGTATCCCCGCCT45340.1485417258425582No Hit
TTGGTCGTGAGAGCCGCTGACAAGCGTGTT42660.13976157971864875No Hit
GTCGCCGCGCTTCGGACCCCAGCGTG42250.13841834840864767No Hit
CGCGTGGTCGTCCGTCGGCCCCTGGC41860.1371406405771832No Hit
TACGAGCCGGGCCTGTGCGAGGTGAAGTT41780.13687854666303667No Hit
CGCCGGTCGTGGCTGGGAACCAC41650.13645264405254853No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGG40270.13193152403352051No Hit
TTTAACACTGAGGCGTGATGACGAGTC39130.12819668575693213No Hit
TGCGTCCTGTCGCGGTGCTGGTTGGTCGG39000.127770783146444No Hit
CGCCGCGCTTCGGACCCCAGC37580.12311861617034268No Hit
CACACGGTTACCCATGTTCTTAATCCCGGG37230.12197195529595153No Hit
ATGTCCTGCCGGTGGCGGTTCGGTCTGGTC37030.12131672051058516No Hit
TGGTCGTCCGTCGGCCCCTGGC36860.12075977094302373No Hit
CCGAGCACGGTCCCGTGCCTAGGCACCC36450.11941653963302265No Hit
GCCGGTCGTGGCTGGGAACCACC36160.1184664491942414No Hit
CGCTTTGCGGGGGTCGTCCGTCGGC35990.11790949962667999No Hit
CTACGAGCCGGGCCTGTGCGAGGTGAAG33200.10876897437081898No Hit
CGCCGGTCGTGGCTGGGAACCACC33160.1086379274137457No Hit
GCCGGTCGTGGCTGGGAACC32600.10680327001471984No Hit
ATCACGGAATTCTTGAACCAAGCGCA32280.10575489435813365No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGA32180.10542727696545046No Hit
CGCCTCCGGGCCGTTCGCTTTGCGGGG31640.10365814304496124No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGGT30840.10103720390349571No Hit
CCTCCGGGCGGGACCTCGAATCGTC30840.10103720390349571No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGC30810.10093891868569076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)