FastQCFastQC Report
Tue 20 Oct 2020
S34.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8855536
Sequences flagged as poor quality0
Sequence length15-30
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACCTCGGGCTGTACGGGACTACCCGG5871996.63086909702586No Hit
AACGACCTCGGGCTGTACGGGACTACCCGG658880.7440317559546933No Hit
GTCCGTCGGCCCCTGGC526700.5947691929658464No Hit
CTTTAACACTGAGGCGTGATGACGAGTC438670.4953624489810668No Hit
CGCCGGTCGTGGCTGGGAACC379480.42852290363903434No Hit
GGGGCTGATTTTGGATTCGACGAAATTT375650.42419792545589563No Hit
GCCTCCGGGCGGGACCTC371870.41992940912893356No Hit
CTCCGGGCGGGACCTCGAATCGTC332610.3755955596589523No Hit
AAGGAACTCCTCATACCCGTTGG290490.3280320920156612No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAG288780.32610109653441643No Hit
ACACCATCGGATGAACCCAGACGGGA278920.31496681849636204No Hit
TCTTTAACACTGAGGCGTGATGACGAGTC276820.31259542053693873No Hit
TTAAAGTGGTACGCGAGCTGGGTTTAGAAC275460.3110596580489312No Hit
TCGTTTCCCGGACGATGCACCA269640.30448749798995794No Hit
ACGTACATCCGAGGGTCGGT250120.2824447893385561No Hit
AATCACGGAATTCTTGAACCAAGCGC238750.269605363243964No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCGGC227690.2571160006576677No Hit
GCCGGTCGTGGCTGGGAACC226370.2556254076546016No Hit
GGGGCTATAGCTCAGACGGGAGAGCGCCT225280.25439453918994853No Hit
GCCGGTCGTGGCTGGGAACCACC214150.24182613000500477No Hit
CGCCGGTCGTGGCTGGGAACCACC213030.24056138442664568No Hit
ATGTCCTGCCGGTGGCGGTTCGGTCTGGTC211410.23873202028651908No Hit
GTCCGTCGGCCCCTG209720.23682360954774503No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGA207980.23485873695279427No Hit
ACGTAACGTTGGCGTC205780.23237441528101743No Hit
TTTAACACTGAGGCGTGATGACGAGTC204910.23139197898354202No Hit
CGCCGGTCGTGGCTGGGAACCAC193830.21888003165477504No Hit
GTCCGTCGGCCCCTGG191670.2164408794679396No Hit
ACGACCTCGGGCTGTACGGGACT191450.21619244730076193No Hit
AACGCGAAAAACCTTACCTACTCTTGACA188470.21282732067262783No Hit
ATGCAAGTCGAGCGGCAGCGGGGAGT185170.20910083816496255No Hit
GGGGACAGAACTAGGAAGCAACTTAAAAGC177960.20095903850427577No Hit
GCCTCCGGGCGGGACCTCGAATCGTC172400.19468048009742153No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCG172310.1945788487563034No Hit
ACGACCTCGGGCTGTACGGGACTACCC164160.18537556619949375No Hit
TCGACTCCTTCTGCCGGCACCA162920.1839753121663104No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG149840.16920489059047358No Hit
AAGTCTCGTCCCAGGCACCA143480.16202294248479143No Hit
TGCAACGCGAAAAACCTTACCTACTC138070.15591376964646747No Hit
GCGGTCCGGGTGATGTCCTC137550.1553265663422293No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCGGCC135080.15253735064709803No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC126390.1427242800435795No Hit
TAGGAACTTCATACCGTGCTCT125380.14158375054880926No Hit
AGCTAGGTTCTCCGGGC123870.13987860249227152No Hit
GTCCGTCGGCCCCTGGCC123310.13924622970309194No Hit
GTAATGATGGCCAGGCTGTCTCCACCCG122090.13786856041237933No Hit
CTCGGTAGTATAGTGGTCAGTATCCCCGCC121320.13699904782725744No Hit
ACGACCTCGGGCTGTACGGGACTACC120350.13590368781742856No Hit
GAAGGCGACCCCCTGGACGAAGACT119120.1345147261554806No Hit
CTCGTAGTATAGTGGTCAGTATCCCCGCCT117210.13235788324952888No Hit
AACGCGAAAAACCTTACCTACTCTTGAC115880.13085599787522742No Hit
CTCCGGGCGGGACCTCGAATCGTCC108550.1225786897597164No Hit
ACGACCTCGGGCTGTACGGGA106420.120173414686587No Hit
GGGTCACGTACATCCGAGGGTCGGT102930.1162323771254501No Hit
GAGGTGTAGAATAAGTGGGAGACGGG101940.11511443237315054No Hit
GCGAAAAACCTTACCTACTCTTGACATCC99810.11270915730002112No Hit
CGTAACGTTGGCGTC98770.11153475069154482No Hit
GGGTGATGTCCTCTTCGGCGGCC98580.11132019563807319No Hit
CACACGGTTACCCATGTTCTTAATCCCGGG96940.10946824675547591No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC96390.10884716633753168No Hit
GTTCAAGTCTCGTCCCAGGCACCA95720.10809057746476328No Hit
AACACTGAGGCGTGATGACGAGTC93720.10583210321769344No Hit
GCGTGGTCGTCCGTCGGCCCCTGGCC93540.10562884053545714No Hit
AACGCGAAAAACCTTACCTACTC90970.10272670112797239No Hit
TGGACGGAGAACTGATAAGGGC90340.10201528174014537No Hit
CGGTCGTGGCTGGGAACC89910.10152970977702536No Hit
GTTTCGGGTCACGTACATCCGAGGGTCGGT89740.10133773946602441No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC89070.10058115059325601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)