FastQCFastQC Report
Wed 21 Oct 2020
S33.no-rRNA.R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.no-rRNA.R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911002
Sequences flagged as poor quality0
Sequence length15-30
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACCTCGGGCTGTACGGGACTACCCGG2144897.368218915686077No Hit
CTCCGGGCGGGACCTCGAATCGTC1018183.497695982345598No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG295871.0163854233009801No Hit
GGGGCTGATTTTGGATTCGACGAAATTT294221.0107172719221766No Hit
AACGACCTCGGGCTGTACGGGACTACCCGG231770.7961863303426105No Hit
GCCTTCACGGGCGTCGCGTCGCGGTCCG198310.6812430908669935No Hit
GGGGACAGAACTAGGAAGCAACTTAAAAGC165480.5684640546451016No Hit
CTACGAGCCGGGCCTGTGCGAGGTGAAGTT151080.5189965517028158No Hit
CGCCGCGCTTCGGACCCCAGCGTG148170.5089999938165621No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC143460.4928199980625228No Hit
GTGCCTTCACGGGCGTCGCGTCGCGGTCCG124570.42792825288337144No Hit
CTCCGGGCGGGACCTCGAATCGTCC113120.3885947175577344No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC111080.38158682130757726No Hit
CGAGCCGGGCCTGTGCGAGGTGAAGTT108690.3733765899164617No Hit
GGTCGGTATTTCCCGCGTCGGCGA85410.29340412682643296No Hit
CTTTAACACTGAGGCGTGATGACGAGTC80420.2762622629596269No Hit
AACACTGAGGCGTGATGACGAGTC75370.2589142844972281No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC71470.24551683578369235No Hit
ACGAGCCGGGCCTGTGCGAGGTGAAGTT70790.24318087036697328No Hit
AAGGAACTCCTCATACCCGTTGG69910.24015785629827804No Hit
GCCTCCGGGCGGGACCTCGAATCGTC65790.22600465406756848No Hit
CGCCGGTCGTGGCTGGGAACC60290.2071108161382232No Hit
CACCCTCCGACACACGTTCCTGACCCTT59420.20412215450212676No Hit
CGAGGCCAGGTGCGTCCTGTCGCGGTGCTG56930.19556839878502313No Hit
GTCGTCCGTCGGCCCCTGGC54880.1885261501022672No Hit
CTCGGTAGTATAGTGGTCAGTATCCCCGCC54450.18704899550051837No Hit
GTCGCCGCGCTTCGGACCCCAGC54170.1860871273877517No Hit
TCTTTAACACTGAGGCGTGATGACGAGTC49660.17059418028568857No Hit
CACCCTCCGACACACGTTCCTGACCCTTT49410.16973536947071832No Hit
CGGAATTCTTGAACCAAGCGCAAGCGG48460.16647188837383142No Hit
GAGGTGTAGAATAAGTGGGAGACGGG45990.15798683752192544No Hit
CTCGTAGTATAGTGGTCAGTATCCCCGCCT45340.1557539294030028No Hit
TTGGTCGTGAGAGCCGCTGACAAGCGTGTT42660.14654747746652183No Hit
GTCGCCGCGCTTCGGACCCCAGCGTG42250.14513902772997064No Hit
CGCGTGGTCGTCCGTCGGCCCCTGGC41860.14379928285861707No Hit
TACGAGCCGGGCCTGTGCGAGGTGAAGTT41780.1435244633978266No Hit
CGCCGGTCGTGGCTGGGAACCAC41650.14307788177404207No Hit
ACGGAATTCTTGAACCAAGCGCAAGCGG40270.13833724607540632No Hit
TTTAACACTGAGGCGTGATGACGAGTC39130.13442106875914203No Hit
TGCGTCCTGTCGCGGTGCTGGTTGGTCGG39000.1339744871353575No Hit
CGCCGCGCTTCGGACCCCAGC37580.12909644170632656No Hit
CACACGGTTACCCATGTTCTTAATCCCGGG37230.1278941065653682No Hit
ATGTCCTGCCGGTGGCGGTTCGGTCTGGTC37030.127207057913392No Hit
CCGAGCACGGTCCCGTGCCTAGGCACCC36450.12521461682266105No Hit
GCCGGTCGTGGCTGGGAACCACC36160.12421839627729557No Hit
CTACGAGCCGGGCCTGTGCGAGGTGAAG33200.11405007622804794No Hit
CGCCGGTCGTGGCTGGGAACCACC33160.1139126664976527No Hit
GCCGGTCGTGGCTGGGAACC32600.11198893027211936No Hit
ATCACGGAATTCTTGAACCAAGCGCA32280.11088965242895744No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGA32180.11054612810296935No Hit
CGCCTCCGGGCCGTTCGCTTTGCGGGG31640.10869109674263362No Hit
CGGAATTCTTGAACCAAGCGCAAGCGGGT30840.10594290213472887No Hit
CCTCCGGGCGGGACCTCGAATCGTC30840.10594290213472887No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGC30810.10583984483693244No Hit
TGCGTCCTGTCGCGGTGCTGGTTGGTCG30330.10419092807218958No Hit
ATCGTCCGCGTGGTCGTCCGTCGGCCCCTG30000.10305729779642886No Hit
CCGTGTGCACCGTGGGGC29810.10240460157705147No Hit
CGTCGAGGGAGGATGGCGACGGATCCGGCC29620.10175190535767409No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCC29150.10013734102553006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)