FastQCFastQC Report
Wed 21 Oct 2020
S34.no-rRNA.R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.no-rRNA.R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8188042
Sequences flagged as poor quality0
Sequence length15-30
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACCTCGGGCTGTACGGGACTACCCGG5871997.171421446055113No Hit
AACGACCTCGGGCTGTACGGGACTACCCGG658880.804685662335391No Hit
CTTTAACACTGAGGCGTGATGACGAGTC438670.5357446871913945No Hit
CGCCGGTCGTGGCTGGGAACC379480.46345634279843706No Hit
GGGGCTGATTTTGGATTCGACGAAATTT375650.45877878984988113No Hit
CTCCGGGCGGGACCTCGAATCGTC332610.40621433060553425No Hit
AAGGAACTCCTCATACCCGTTGG290490.3547734611033993No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAG288780.3526850497347229No Hit
ACACCATCGGATGAACCCAGACGGGA278920.340643098801887No Hit
TCTTTAACACTGAGGCGTGATGACGAGTC276820.33807838308596855No Hit
TTAAAGTGGTACGCGAGCTGGGTTTAGAAC275460.33641742433661187No Hit
TCGTTTCCCGGACGATGCACCA269640.32930949792392367No Hit
ACGTACATCCGAGGGTCGGT250120.30546985469786303No Hit
AATCACGGAATTCTTGAACCAAGCGC238750.29158375103596196No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCGGC227690.27807624826545835No Hit
GCCGGTCGTGGCTGGGAACC226370.2764641412440239No Hit
GGGGCTATAGCTCAGACGGGAGAGCGCCT225280.27513293165814245No Hit
GCCGGTCGTGGCTGGGAACCACC214150.26153993836377487No Hit
CGCCGGTCGTGGCTGGGAACCACC213030.26017208998195174No Hit
ATGTCCTGCCGGTGGCGGTTCGGTCTGGTC211410.2581935950011004No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGA207980.254004559331767No Hit
ACGTAACGTTGGCGTC205780.25131771429604294No Hit
TTTAACACTGAGGCGTGATGACGAGTC204910.2502551892137339No Hit
CGCCGGTCGTGGCTGGGAACCAC193830.23672326057926915No Hit
ACGACCTCGGGCTGTACGGGACT191450.23381658276789494No Hit
AACGCGAAAAACCTTACCTACTCTTGACA188470.2301771290376869No Hit
ATGCAAGTCGAGCGGCAGCGGGGAGT185170.22614686148410085No Hit
GGGGACAGAACTAGGAAGCAACTTAAAAGC177960.2173413375261143No Hit
GCCTCCGGGCGGGACCTCGAATCGTC172400.2105509473449208No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCG172310.2104410309570957No Hit
ACGACCTCGGGCTGTACGGGACTACCC164160.20048749139293617No Hit
TCGACTCCTTCTGCCGGCACCA162920.19897308782734624No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG149840.18299857279676876No Hit
AAGTCTCGTCCCAGGCACCA143480.17523114805713014No Hit
TGCAACGCGAAAAACCTTACCTACTC138070.1686239518556451No Hit
GCGGTCCGGGTGATGTCCTC137550.16798887939265578No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCGGCC135080.16497228519345652No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGC126390.15435924730234651No Hit
TAGGAACTTCATACCGTGCTCT125380.15312574117230957No Hit
AGCTAGGTTCTCCGGGC123870.1512815884432444No Hit
GTAATGATGGCCAGGCTGTCTCCACCCG122090.14910768655070405No Hit
CTCGGTAGTATAGTGGTCAGTATCCCCGCC121320.14816729078820065No Hit
ACGACCTCGGGCTGTACGGGACTACC120350.14698263638608594No Hit
GAAGGCGACCCCCTGGACGAAGACT119120.1454804457524766No Hit
CTCGTAGTATAGTGGTCAGTATCCCCGCCT117210.14314777574418888No Hit
AACGCGAAAAACCTTACCTACTCTTGAC115880.1415234557907739No Hit
CTCCGGGCGGGACCTCGAATCGTCC108550.1325713766490206No Hit
ACGACCTCGGGCTGTACGGGA106420.1299700221371605No Hit
GGGTCACGTACATCCGAGGGTCGGT102930.1257077088759437No Hit
GAGGTGTAGAATAAGTGGGAGACGGG101940.1244986286098679No Hit
GCGAAAAACCTTACCTACTCTTGACATCC99810.12189727409800781No Hit
CGTAACGTTGGCGTC98770.12062712917202917No Hit
GGGTGATGTCCTCTTCGGCGGCC98580.12039508346439844No Hit
CACACGGTTACCCATGTTCTTAATCCCGGG96940.11839216261958597No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCC96390.11772045136065497No Hit
GTTCAAGTCTCGTCCCAGGCACCA95720.11690218491795719No Hit
AACACTGAGGCGTGATGACGAGTC93720.11445959852184441No Hit
GCGTGGTCGTCCGTCGGCCCCTGGCC93540.11423976574619427No Hit
AACGCGAAAAACCTTACCTACTC90970.11110104222718936No Hit
TGGACGGAGAACTGATAAGGGC90340.11033162751241384No Hit
CGGTCGTGGCTGGGAACC89910.1098064714372496No Hit
GTTTCGGGTCACGTACATCCGAGGGTCGGT89740.10959885159357999No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGC89070.10878058515088222No Hit
AATGATGGCCAGGCTGTCTCCACCCGAGA88340.10788904111630107No Hit
GATAAACCGGAGGAAGGCGGGGATAACGTC86780.1059838237273331No Hit
CGCGAAAAACCTTACCTACTCTTGACATCC85750.10472589173333502No Hit
CACGTAACGTTGGCGTC83440.10190470444582478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)