FastQCFastQC Report
Tue 27 Oct 2020
S33.only-adapter-trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.only-adapter-trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510044
Sequences flagged as poor quality0
Sequence length0-72
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCCGTCGCCGGTGAAATACCACTACTCTCATCGTTTCCTTACTTACTCGATGAAGCGGAACGCGTG373247.317800032938335No Hit
CATATCACGTTGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCGTGGATAGTACT214554.206499831387096No Hit
TCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG151892.977978370493526No Hit
AAGGAACTCCTCATACCCGT87171.7090682372501196No Hit
CAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG78851.5459450557206829No Hit
CTGCAAAGAGGGGACGGAGAAAGCTAGGTTCTCCGGGCGACGGTCGTCCCGGTTTAAGCGTAAAGG78721.5433962560092855No Hit
CGTCGCCGGTGAAATACCACTACTCTCATCGTTTCCTTACTTACTCGATGAAGCGGAACGCGTG72631.423994792606128No Hit
GCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC69201.3567456925284878No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCA41880.821105630102501No Hit
ATATCACGTTGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCGTGGATAGTACT35950.704841150959525No Hit
GTCAACTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC33760.6619036788982912No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCT32840.6438660194022476No Hit
AGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC30910.6060261467638086No Hit
CGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC27280.5348558163609414No Hit
AACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG26270.5150536032185458No Hit
TGTCAACTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC24250.4754491769337547No Hit
CCACGTTCCCG24220.4748609923849707No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTC24050.4715279466085279No Hit
CTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC23240.45564696379135916No Hit
GGTCTCATGGTGTAACGGTTAGCACTC22990.45074542588482563No Hit
GTCCGTCGGCCCC21890.429178659096078No Hit
TGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC18960.37173263483150476No Hit
CGCTGTGGGATGAACCAAACGCCGAGCTACGGCGACTAAATCGACGCTCAT17530.3436958380061328No Hit
CTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG17070.33467700825811103No Hit
CAACTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC16970.33271639309549766No Hit
GTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC15870.31114962630675No Hit
GGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC15040.2948765204570586No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCATGTTTCG14690.28801436738791164No Hit
CAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG14650.28723012132286624No Hit
CGCCGCGCTTCGGACCCCAGCG14060.27566249186344705No Hit
TTGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCG14050.27546643034718576No Hit
ACCTCGAATCGTCCGCGTGGTCGTCCGTCGGCCCC14040.2752703688309244No Hit
CTCGACGGCGGTGTCAACTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC13630.2672318466642094No Hit
CTGATGACTGCGGCAACCGTCGTTGCGTTAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCGGACATT12960.2540957250746994No Hit
TGCAAAGAGGGGACGGAGAAAGCTAGGTTCTCCGGGCGACGGTCGTCCCGGTTTAAGCGTAAAGG12850.25193904839582465No Hit
GTCGCCGCGCTTCGGACCCCAGCG12620.2474296335218138No Hit
ACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG12360.24233203409901893No Hit
TCCCATGTGGTCTACTGGCTAGGATATC12080.23684231164370134No Hit
CGTCCGTCGGCCCC10940.21449129878990833No Hit
CATATCACGTTGAATACACCAGTTCTCGTCCGAACACTGAAGTTAAGCAACGTCGAGCGTGGATAGTACT10910.2139031142411243No Hit
ATAACGAACGAGACTCTTTCCTGCTAACTGGGCGTTTTTGGCGATCGACTCCGCCGGTCGTGGC10780.21135431452972683No Hit
CGGTCGCCGCGCTTCGGACCCCAGCG10660.20900157633459074No Hit
CCTCGAATCGTCCGCGTGGTCGTCCGTCGGCCCCTGGCCGCGAGGCCAGGTGCGTCCTGTCGCGGTGCTGGT10210.20017880810283034No Hit
CGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCA10000.19606151626134216No Hit
CACCACGTTCCCG9410.18449388680192297No Hit
ATGAACCAAACGCCGAGCTACGGCGACTAAATCGACGCTCAT9310.18253327163930955No Hit
ACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCT9170.17978841041165075No Hit
TGAACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG9130.17900416434660538No Hit
CGTTCCCG9100.17841597979782137No Hit
GGTGATTGTAGCTCAGTTGGTAGAGCCC8790.17233807279371977No Hit
ACGACCTCGGGCTGTACGGGACTACCCGG8630.16920108853353827No Hit
CGTTCGCGAGACGAGAGGTGCGCCCAGAGCACACACGCTAGGACCCGAAAGA8470.1660641042733568No Hit
TATTGCACTTGTCCCGGCC8410.16488773517578875No Hit
CCTTGTGAACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG8280.1623389354643913No Hit
TAACGAACGAGACTCTTTCCTGCTAACTGGGCGTTTTTGGCGATCGACTCCGCCGGTCGTGGC8270.16214287394812996No Hit
AAGGGAAGTCGGCAAAATCGATCCGTAACTTCGGAATAAGGATTGGCTCTGAGGAACGTGGTCCGTCGGGCT8210.1609665048505619No Hit
ACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCA7920.15528072087898298No Hit
GGAATACCAGTGGCGAAGGCGACCCCCTGGACGAAGACTGACGCTCAAGTACGAAAGCG7850.1539082902651536No Hit
CATATCACGTTGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCGTGGACAGTACT7450.1460658296146999No Hit
TTCCTTGTGAACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG7220.14155641474068903No Hit
TTCGGTCGCCGCGCTTCGGACCCCAGCG7150.14018398412685965No Hit
CGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGCTGTGAAATGT7090.1390076150292916No Hit
GCAATCCGGGCCGCCTGAATACTGTGTGCATAGAATAACTGAATAGGACGTCGGTTCCAGTTCGAGGTTAAG7000.1372430613829395No Hit
GCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTCTGGTCGACTT6970.13665487683415548No Hit
TAACAGCTGATCTGGTGACTGCTCGCTCAGTGTTGCCAAATGCCCCAAAATCCCCAAA6730.13194940044388329No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCT6620.1297927237650085No Hit
TGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCG6500.1274399855698724No Hit
AAAGAGGGGACGGAGAAAGCTAGGTTCTCCGGGCGACGGTCGTCCCGGTTTAAGCGTAAAGG6300.12351875524464556No Hit
GGTCCCATGGTGTAACGGTTAGCACTC6210.12175420159829349No Hit
GGCCCCCGCTACAAGCCCAATGCCGATTGGCGGACACGTTTCGGGCTC6060.11881327885437334No Hit
AGACGAGAGGTGCGCCCAGAGCACACACGCTAGGACCCGAAAGA6010.11783297127306665No Hit
CGTGCCCGTCGCCGGTGAAATACCACTACTCTCATCGTTTCCTTACTTACTCGATGAAGCGGAACGCGTG5740.11253931033401039No Hit
CGCGAGACGAGAGGTGCGCCCAGAGCACACACGCTAGGACCCGAAAGA5570.10920626455756757No Hit
CTCTCACCCGGGAGGCCCGGGTTCAATTCCCGGCA5520.10822595697626088No Hit
CTCGACGGCGGTGTCAACTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTCTGGTCGACTT5520.10822595697626088No Hit
CGCGTGGTCGTCCGTCGGCCCCTGGCCGCGAGGCCAGGTGCGTCCTGTCGCGGTGC5460.10704958787869283No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGC5400.10587321878112477No Hit
CGAGACGAGAGGTGCGCCCAGAGCACACACGCTAGGACCCGAAAGA5340.10469684968355672No Hit
CTCCGGGCGGGACCTCGAATCGTCCGCGTGGTCGTCCGTCGGCCCC5320.10430472665103403No Hit
GTGTCAACTGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC5200.10195198845589792No Hit
GAAACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC5170.1013638039071139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)