FastQCFastQC Report
Wed 21 Oct 2020
S33.trimmed.AACTGTAGGCACCATCAAT.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.trimmed.AACTGTAGGCACCATCAAT.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076405
Sequences flagged as poor quality0
Sequence length1-73
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAATGATGATGTTTTACTCAAGGCATGTATTAGCTGAGCTCTCGAGCGTTGGTGAAACGTAAACCTGTCTG866988.054403314737483No Hit
CTGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAAACCCTCTTTCATGTATTAGCTCTAACTG471984.384780821345126No Hit
TGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAAACCCTCTTTCATGTATTAGCTCTAACTG461974.2917860842340945No Hit
CGCGCCTCCGGGCCGTTCGCTTTGCGGGGGTCGTCCGTCGGCTTCGCC456384.239853958314947No Hit
TGTAATGATGATGCGTTCCGTCTGACATCTCCCTGATGTTGCAACCCGTTTACATGTATTAGCTCTAACTG423483.9342069202577092No Hit
TGTAATGATGATGCGTTCCGTCTGACTTCTCCCTGATGTTGCAACCCGTTTACATGTATTAGCTCTAACTG325173.0208889776617536No Hit
TTCTATGATGTTAAAAATTACCGTTTGACAGACACAACAAATATGATAATACCTTTTGGCTGCTTTATTTATG309522.875497605455196No Hit
ACCCCGAACGGTGGATCACTTGGCTCGTGGGTCGACGAAGAACGCAGCA228372.1215992121924367No Hit
TGTAATGATGATGCGTTCCATCTGACATCTCGCTGATGTTGCAACCCGTTTACATGTATTAGCTCTAACTG211481.964688012411685No Hit
AGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACAGAGTTCCGACCGCCTCGTGCGGGAGG164961.532508674708869No Hit
TTGTCGATGATGAATAAATGACACCTCTTGTATGAGCCCGGATGATTTATACAAAGATTTTCCATGCACTACC154861.4386778210803555No Hit
GTACATGATGAAACAACAGAATTCGCAGAACTTGAAGCTTCGTGCTTTGATTTTTGTTGATAAAA106500.9894045456868No Hit
TTCGATGATGTTAAAAATTACCGTTTGACAGACACAACAAACATGATAATACCTTTTGGCTGCTTTTTTTATA90670.8423409404452785No Hit
TGTAATGATGCGTTTTTATATTTCGTCTCCAAGATTGACAAAGGTCTCTGAGGAAATTCATCCGACTG82050.762259558437577No Hit
TGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAAACCTTCTTTCATGTATTAGCTCTAACTG81580.7578931721796164No Hit
GTACATGATGAAACAACAGAATTCGCAGAACTTGAAGTCTCGTGCTTTGATTTTTGTTGATAAAA78980.7337386950079199No Hit
CTGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAAACCTTCTTTCATGTATTAGCTCTAACTG68270.6342408294275853No Hit
CTGTAATGATGTTTTACTCAAGGCATGTATTAGCTGAGCTCTCGAGCGTTGGTGAAACGTAAACCTGTCTG68240.6339621239217581No Hit
GGAGATGATGACTGGGGTATGCATGCGACTGTGTGAAGCCTACGCCTCATCCTTGTTACGATTTGGTAGGCGA63290.5879757154602588No Hit
TGTAATGATGTTTTACTCAAGGCATGTATTAGCTGAGCTCTCGAGCGTTGGTGAAACGTAAACCTGTCTG62220.5780352190857531No Hit
GGAGATGATGACTGGGGTATACATGCGTCTGTGTGAAGCCTACGCCTCATCCTTGTTACGATTTGGTAGGCGA59710.5547168584315383No Hit
CTCCGGGCCGTTCGCTTTGCGGGGGTCGTCCGTCGGCTTCGCC56090.5210863940617148No Hit
TTGTAATGATGCGTTTTTATATTTCGTCTCCAAGATTGACAAAGGTCTCTGAGGAAATTCATCCGACTG54620.5074298242761786No Hit
CGCCTCCGGGCCGTTCGCTTTGCGGGGGTCGTCCGTCGGCTTCGCC54420.5055717875706635No Hit
GTACATGATGAAACAACAGAATTCGCAGAACTTGAAGCCTTGTGCTTTGATTTTTGTTGATAAAA47000.4366386257960526No Hit
TACGAGTAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGCAGCA41720.3875864567704535No Hit
CGTGCGGGAGGAACGCTTTTATTAGATCAA37320.3467096492491209No Hit
TGTACATGATGAAACAACAGAATTCGCAGAACTTGAAGCTTCGTGCTTTGATTTTTGTTGATAAAA35440.3292441042172788No Hit
TGTAATGATGCGTTCTTATATTTCGTCTCCAAGATTGACAAAGGTCTCTGAGGAAATTCATCCGACTG35300.3279434785234182No Hit
TTGTAATGATGATGCGTTCCGTCTGACATCTCCCTGATGTTGCAACCCGTTTACATGTATTAGCTCTAACTG34150.3172597674667063No Hit
CTAGGTGAAACCGCGAAAGGCTCATTAAATCAGTTATGGTTCCTTGGATCGTACCACTCAGTTACTTGGAT34100.3167952582903275No Hit
ATGTAATGATGCGTTTTTATATTTCGTCTCCAAGATTGACAAAGGTCTCTGAGGAAATTCATCCGACTG32060.29784328389407333No Hit
TGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAAGCCCTCTTTCATGTATTAGCTCTAACTG29120.2705301443230011No Hit
ACGACCTCGGGCTGTACGGGACTACCCGGTGGATTTAAGCATATCGATAAGCGGAGGAAAAGA26990.2507420534092651No Hit
ACGAGTAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGCAGCA26310.2444247286105137No Hit
GCCGGCGTAGCTCAGTTGGTAGAGC25080.23299780287159574No Hit
TTCGATGATGTTAAAAATTACCGTTTGACAGACACAACAAACATGATAATACCCTTTGGCTGCTTTTTTTATA25010.23234749002466545No Hit
TTGTCTATGATGAATAAATGACACCTCTTGTATGAGCCCGGATGATTTATACAAAGATTTTCCATGCACTACC23280.2162754725219597No Hit
CGAGAGGTGCGCCCAGAGCACACACGCTAGGACCCGAAAGATGGTG22060.20494144861831748No Hit
TGTACATGATGAAACAACAGAATTCGCAGAACTTGAAGTCTCGTGCTTTGATTTTTGTTGATAAAA21560.20029635685452968No Hit
GTCCGATCACTGAAGTTAAGCAACGTCGAGCGTGGATAGTACTTGGATGGGTGACCGCTTGGGAACACCACGT21370.1985312219842903No Hit
TTGTCGATGATGAATAAATGACACCTCTTGTATGAGCCCAGATGATTTATACAAAGATTTTCCATGCACTACC21050.19555836325546613No Hit
TTACGAGTAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGCAGCA20440.189891351303645No Hit
TGTCGATGATGAATAAATGACACCTCTTGTATGAGCCCGGATGATTTATACAAAGATTTTCCATGCACTACC20260.1882191182686814No Hit
TTGTAATGATGATGCGTTCCATCTGACATCTCGCTGATGTTGCAACCCGTTTACATGTATTAGCTCTAACTG20240.18803331459812989No Hit
TGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAACCCCTCTTTCATGTATTAGCTCTAACTG19380.1800437567644149No Hit
CTGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAACCCCTCTTTCATGTATTAGCTCTAACTG19300.17930054208220883No Hit
TTGTAATGATGATGCGTTCCGTCTGACTTCTCCCTGATGTTGCAACCCGTTTACATGTATTAGCTCTAACTG17590.16341432825005459No Hit
TTTCTATGATGTTAAAAATTACCGTTTGACAGACACAACAAACATGATAATACCTTTTGGCTGCTTTTTTATA17480.16239240806202127No Hit
CAACTGGAGGACCGAACCGACTAATGTTGAAAAATTAGCGGATGACTTGTGGCTAGGGGTGAAAGGCCAATCA17470.1622995062267455No Hit
CTGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGAAGCCCTCTTTCATGTATTAGCTCTAACTG16770.1557963777574426No Hit
TTGTCGATGATGAATAAATGACACCTCTTGTATGAGTCCGGATGATTTATACAAAGATTTTCCATGCACTACC16680.1549602612399608No Hit
TGTTGTGATGAAGAGACTATTCCGATGTTTGTCAGTGACGTGACACCCTCTTTCATGTATTAGCTCTAACTG16620.15440285022830627No Hit
GCGCCTCCGGGCCGTTCGCTTTGCGGGGGTCGTCCGTCGGCTTCGCC16480.15310222453444566No Hit
CGAGTAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGCAGCA15100.14028177126639138No Hit
TGTACATGATGAAACAACAGAATTCGCAGAACTTGAAGCCTTGTGCTTTGATTTTTGTTGATAAAA14250.13238511526795213No Hit
CATAACCCTTGGCAGGGGATCACTAGGCTCATGGATCGATGAAGACCGCAGCTAAATGCGCGTCACAATGTG13410.12458136110478862No Hit
GTACATGATGAAACAACAGAATTCGCAGAACTCGAAGCTTCGTGCTTTGATTTTTGTTGATAAAA12280.11408345371862821No Hit
CGCTTTGCGGGGGTCGTCCGTCGGCTTCGCC12100.11241122068366462No Hit
TTAAAGTGGTACGCGAGCTGGGTTTAG11330.1052577793674314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)