FastQCFastQC Report
Tue 27 Oct 2020
S34.only-adapter-trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.only-adapter-trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013014
Sequences flagged as poor quality0
Sequence length0-72
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC20699820.43387356936824No Hit
AAGGAACTCCTCATACCCGT350643.4613539398270903No Hit
GTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC252212.4896990564789827No Hit
AGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC239192.3611717113485104No Hit
CCACGTTCCCG214532.1177397350875706No Hit
GTCCGTCGGCCCC196931.9440007739280996No Hit
TCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG164971.6285066149135157No Hit
CTGCAAAGAGGGGACGGAGAAAGCTAGGTTCTCCGGGCGACGGTCGTCCCGGTTTAAGCGTAAAGG154071.5209069173772525No Hit
GAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC138541.3676020272177876No Hit
CTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC121171.196133518391651No Hit
GAAACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC120331.18784143160904No Hit
CGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC96800.9555642863770886No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCA91600.9042323205799723No Hit
CGTTCCCG91500.9032451673915661No Hit
CAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG87830.8670166453770629No Hit
GGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC68890.6800498314929507No Hit
TATTGCACTTGTCCCGGCC67810.6693885770581651No Hit
CACCACGTTCCCG63900.6307908873914871No Hit
AACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC62890.6208206401885857No Hit
AGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGGAGAGATC61450.6066056342755382No Hit
GGAATACCAGTGGCGAAGGCGACCCCCTGGACGAAGACTGACGCTCAAGTACGAAAGCG61040.6025583062030733No Hit
ACGTTCCCG59890.5912060445364032No Hit
TGCAAAGAGGGGACGGAGAAAGCTAGGTTCTCCGGGCGACGGTCGTCCCGGTTTAAGCGTAAAGG58960.5820255198842267No Hit
TCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC49120.4848896461450681No Hit
ACCACGTTCCCG48680.4805461721160813No Hit
GGTGATTGTAGCTCAGTTGGTAGAGCCC46840.4623825534494094No Hit
GAAGGCGACCCCCTGGACGAAGACTGACGCTCAAGTACGAAAGCG46780.46179026153636576No Hit
AAACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC45980.4538930360291171No Hit
GTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGC45290.4470816790291151No Hit
ACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC44430.43859216160882275No Hit
AGTTCGGTCCCTATCTGCCGTGGGCGC41970.41430819317403317No Hit
GAAACCCATAAAGTGCGTCGTAGTCCGGAC41570.41035958042040876No Hit
ACCCCGAACGGTGGATCACT37340.36860300055083145No Hit
CGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGC37090.36613511757981626No Hit
CATATCACGTTGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCGTGGATAGTACT35360.34905736742039106No Hit
GACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC35360.34905736742039106No Hit
GAGAGGACGATCAGCCACAC33130.3270438513189354No Hit
GTTCCCG32750.32329266920299227No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAG31900.31490186710154056No Hit
CCACCACGTTCCCG28600.2823258118841398No Hit
CGCCGGTCGTGGC27010.26663007618848306No Hit
ACGACCTCGGGCTGTACGGGACTACCCGG26700.26356990130442426No Hit
TAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC26210.2587328506812344No Hit
CGTCCGTCGGCCCC25420.25093434049282637No Hit
CGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGCTGTGAAATGT25060.24738058901456447No Hit
CTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG24640.24323454562325894No Hit
CACGTTCCCG22210.21924672314499108No Hit
AACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC22020.21737113208701955No Hit
TCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGCTGTGAAATGT21660.21381738060875763No Hit
GTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC20640.20374841808701558No Hit
GCGCAACCCTCATCCTTTTTTGCCAGCGATTCGGTCGGGAACTCAAAGGAGACTGCCGGT20300.2003920972464349No Hit
GAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC20220.19960237469571004No Hit
ACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG20130.19871393682614455No Hit
GTTCGGTCCCTATCTGCCGTGGGCGC19160.18913855089860557No Hit
ACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTCA19000.18755910579715582No Hit
CGCGTGCCCGTCGCCGGTGAAATACCACTACTCTCATCGTTTCCTTACTTACTCGATGAAGCGGAACGCGTG18560.18321563176816905No Hit
AACGCGAAAAACCTTACCTACTCTTGACATCCAGAGAATC17980.17749014327541376No Hit
CGGCACGTAGCGCAGTCTGGTAGCGCACCGGCA17860.17630555944932647No Hit
ACCAGTGGCGAAGGCGACCCCCTGGACGAAGACTGACGCTCAAGTACGAAAGCG17530.17304795392758637No Hit
ATATCACGTTGAATACACCAGTTCTCGTCCGATCACTGAAGTTAAGCAACGTCGAGCGTGGATAGTACT17420.1719620854203397No Hit
GAAACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC17310.17087621691309302No Hit
GCCCTCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGCTGTGAAATGT17150.16929677181164327No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCTCTC17090.16870447989859963No Hit
GTGCCCTCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGCTGTGAAATGT16600.16386742927540981No Hit
AACAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG15770.15567405781163934No Hit
GTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGC15180.14984985400004344No Hit
TAACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAG14630.14442051146381No Hit
TGAGAGGACGATCAGCCACAC14470.14284106636236024No Hit
CTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC14300.1411629059420699No Hit
AGTGGCGAAGGCGACCCCCTGGACGAAGACTGACGCTCAAGTACGAAAGCG14090.13908988424641713No Hit
GCTGATATAGCTCAGTCGGTAGAGCGCGCCCT13870.13691814723192375No Hit
GGTCGTCCGTCGGCCCC13860.13681943191308316No Hit
CCTCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGCTGTGAAATGT13810.13632585531888008No Hit
AGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC13590.1341541183043867No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC13120.12951449831887812No Hit
CTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC12890.12724404598554415No Hit
GGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC12890.12724404598554415No Hit
ACCCCGAACGGTGGATCACTTGGCTCG12650.12487487833336952No Hit
TGGCGCGTCAGCGAAATGTCCTGCCGGTGGCGGTTCGGTC12590.12428258642032589No Hit
AACCGCCACGGACGAAAGGGGACGCGGTCCCCATTCCGCTACCTGGACGCGGAACCGCT12470.12309800259423859No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCC12280.12122241153626702No Hit
AGGCGACCCCCTGGACGAAGACTGACGCTCAAGTACGAAAGCG11770.11618793027539599No Hit
CGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC11360.11214060220293105No Hit
CGAAACTAACAGACTAGAGTCTCGTAGAGGAGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATC11290.11144959497104678No Hit
CAGCCGTTGCACAAGGCTCAGTCGATCCTAAGTCCTCGCCAAACAGCGATGCTATAAGCAG11240.11095601837684377No Hit
AACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTT11240.11095601837684377No Hit
GCAACCCTCATCCTTTTTTGCCAGCGATTCGGTCGGGAACTCAAAGGAGACTGCCGGT10860.10720483626090063No Hit
ACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAG10800.10661254434785698No Hit
ATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCAATGGTAGTG10650.10513181456524787No Hit
TTGTACACACCGCCCGTCACACCA10550.10414466137684178No Hit
AACGTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAG10320.10187420904350779No Hit
GGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTCGAGATC10210.1007883405362611No Hit
ATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCAA10160.10029476394205807No Hit
CTTGTACACACCGCCCGTCACACCA10150.10019604862321745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)