FastQCFastQC Report
Fri 29 Apr 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length15-17
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAAAAGAACGT6573.03370786516854No Hit
GACCACGAACGTGCA1112.359550561797752No Hit
AATGGAAATCTCTCC33.3707865168539324No Hit
TTAAAAAAAAGAACGT33.3707865168539324No Hit
ATTCCTCTACCTCTA11.1235955056179776No Hit
TTAAAAAAAAGAACG11.1235955056179776No Hit
AAAAAAAAATTTTAAAA11.1235955056179776No Hit
TGAAATGATGATTTCA11.1235955056179776No Hit
TTCAAATGATGATTT11.1235955056179776No Hit
AAAAAAAAGAACGTG11.1235955056179776No Hit
AAAAAAAAATTTTAA11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)