FastQCFastQC Report
Fri 29 Apr 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576
Sequences flagged as poor quality0
Sequence length15-35
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAATTGAAAACGA221.3959390862944163No Hit
CGTCGCCTACACTCAA181.1421319796954315No Hit
GTCGTCGCCTACACTCAA150.951776649746193No Hit
GGCCACGCGCGCTCG120.7614213197969544No Hit
GGCCACGCGCGCTCGGGAC100.6345177664974619No Hit
ATTGTCGTCGCCTACAC100.6345177664974619No Hit
GTCGTCGCCTACACTCAAT90.5710659898477157No Hit
TCGTCGCCTACACTCAA90.5710659898477157No Hit
GGCCACGCGCGCTCGGG90.5710659898477157No Hit
GGTATTGTCGTCGCCTACAC90.5710659898477157No Hit
GGCCACGCGCGCTCGGGACTCC80.5076142131979695No Hit
TGTCGTCGCCTACAC80.5076142131979695No Hit
GGTATTGTCGTCGCC80.5076142131979695No Hit
GCCACGCGCGCTCGGGACTCC70.4441624365482234No Hit
GGTATTGTCGTCGCCTA70.4441624365482234No Hit
TCGTCGCCTACACTCA70.4441624365482234No Hit
GGTATTGTCGTCGCCTACACTCAA70.4441624365482234No Hit
GGGACTCCTGGTATTGTCGTCGCC70.4441624365482234No Hit
GGCCACGCGCGCTCGGGACTCCTGGT60.3807106598984772No Hit
CTGTACACCACTACGAT60.3807106598984772No Hit
ACACTCAATTCAGTCT60.3807106598984772No Hit
TTGTCGTCGCCTACACTCA60.3807106598984772No Hit
GTCGTCGCCTACACTCA60.3807106598984772No Hit
GGACTCCTGGTATTGTCGTCGCCTACACTCAA60.3807106598984772No Hit
TATTGTCGTCGCCTACACTCAAT60.3807106598984772No Hit
TCGCCTACACTCAAT60.3807106598984772No Hit
GTCGCCTACACTCAA60.3807106598984772No Hit
CGCGCGCTCGGGACTCC60.3807106598984772No Hit
GTATTGTCGTCGCCTACAC60.3807106598984772No Hit
ATTGTCGTCGCCTACACTCAA60.3807106598984772No Hit
GGTATTGTCGTCGCCTACA60.3807106598984772No Hit
CACTACGATTAGAAC50.31725888324873097No Hit
GCCACGCGCGCTCGGG50.31725888324873097No Hit
GGTATTGTCGTCGCCTACACT50.31725888324873097No Hit
GTATTGTCGTCGCCTACA50.31725888324873097No Hit
CGCCTACACTCAATTCAG50.31725888324873097No Hit
CGCGCTCGGGACTCC50.31725888324873097No Hit
CGTCGCCTACACTCAAT50.31725888324873097No Hit
GTATTGTCGTCGCCTACACTCAA50.31725888324873097No Hit
TGTCGTCGCCTACACTC50.31725888324873097No Hit
GTCGTCGCCTACACTC50.31725888324873097No Hit
GGTATTGTCGTCGCCTACACTCAAT50.31725888324873097No Hit
AGGCGTTCAGGCGTAA50.31725888324873097No Hit
TATTGTCGTCGCCTACAC50.31725888324873097No Hit
TCACGTGACCGCGAG50.31725888324873097No Hit
GGCCACGCGCGCTCGG50.31725888324873097No Hit
CGGGACTCCTGGTATTGTCGTCGCCTACAC50.31725888324873097No Hit
GATTAGAACGGATAC40.25380710659898476No Hit
TTAGAGGCGTTCAGG40.25380710659898476No Hit
GCCACGCGCGCTCGGGAC40.25380710659898476No Hit
GGACTCCTGGTATTGTCGTCGCCTACAC40.25380710659898476No Hit
ATTGTCGTCGCCTACACTCAATTCAG40.25380710659898476No Hit
ACTCCTGGTATTGTCGTCGCCTACAC40.25380710659898476No Hit
GGCCACGCGCGCTCGGGACTCCT40.25380710659898476No Hit
TATTGTCGTCGCCTACACT40.25380710659898476No Hit
GGTATTGTCGTCGCCTACACTC40.25380710659898476No Hit
GCCACGCGCGCTCGGGACTCCTGGTA40.25380710659898476No Hit
CGCGCTCGGGACTCCTGGTATTGTCG40.25380710659898476No Hit
TCAGTCTGTACACCAC40.25380710659898476No Hit
CGCCTACACTCAATTCA40.25380710659898476No Hit
GGGCCACGCGCGCTCGGG40.25380710659898476No Hit
TGGTATTGTCGTCGCCTACACTCAA40.25380710659898476No Hit
TACACTCAATTCAGTCTGTACAC40.25380710659898476No Hit
TCAGTCTGTACACCACTACGATTAGAACGGATACG40.25380710659898476No Hit
GGCCACGCGCGCTCGGGA40.25380710659898476No Hit
CGCCTACACTCAATT40.25380710659898476No Hit
ATTGTCGTCGCCTACACTCAAT40.25380710659898476No Hit
TACACCACTACGATTA40.25380710659898476No Hit
GGGACTCCTGGTATTGTCGTC40.25380710659898476No Hit
GGTATTGTCGTCGCCTACACTCAATT40.25380710659898476No Hit
GGACTCCTGGTATTGTCGTCG40.25380710659898476No Hit
TTGTCGTCGCCTACACTCAA40.25380710659898476No Hit
CCACGCGCGCTCGGG40.25380710659898476No Hit
TATTGTCGTCGCCTACACTCAA40.25380710659898476No Hit
GTCGCCTACACTCAATT30.1903553299492386No Hit
CTGGTATTGTCGTCGCCTACACTCAA30.1903553299492386No Hit
TGTCGTCGCCTACACT30.1903553299492386No Hit
GCTCGGGACTCCTGG30.1903553299492386No Hit
CGGGACTCCTGGTATTGTCGTC30.1903553299492386No Hit
GCGCTCGGGACTCCTGGTATTGTCGTC30.1903553299492386No Hit
CTGTACACCACTACGATTAGAAC30.1903553299492386No Hit
GGCCACGCGCGCTCGGGACTC30.1903553299492386No Hit
CCACGCGCGCTCGGGACT30.1903553299492386No Hit
TCTGTACACCACTACGA30.1903553299492386No Hit
GTCGTCGCCTACACT30.1903553299492386No Hit
GCCACGCGCGCTCGG30.1903553299492386No Hit
GGTATTGTCGTCGCCTACACTG30.1903553299492386No Hit
GTACACCACTACGATTAGAAC30.1903553299492386No Hit
CGGGACTCCTGGTATTGTCGTCGCCTACACTCAA30.1903553299492386No Hit
GTATTGTCGTCGCCTAC30.1903553299492386No Hit
CCTACACTCAATTCA30.1903553299492386No Hit
CCTGGTATTGTCGTCGCCTACACTCAA30.1903553299492386No Hit
GGACTCCTGGTATTGTCGTCGCC30.1903553299492386No Hit
GGTATTGTCGTCGCCTACACTCAATTCAGT30.1903553299492386No Hit
CTACACTCAATTCAG30.1903553299492386No Hit
GACTCCTGGTATTGT30.1903553299492386No Hit
GCGCTCGGGACTCCT30.1903553299492386No Hit
TCTGTACACCACTAC30.1903553299492386No Hit
GTACACCACTACGATTAGAACGGATA30.1903553299492386No Hit
GGTATTGTCGTCGCCTACACTCAATTCA30.1903553299492386No Hit
AAAGTAAATCACACA30.1903553299492386No Hit
GTATTGTCGTCGCCTACACT30.1903553299492386No Hit
TGTCGTCGCCTACACTCAA30.1903553299492386No Hit
TCGCCTACACTCAATTCA30.1903553299492386No Hit
AGAACGGATACGGCCT30.1903553299492386No Hit
CCACGCGCGCTCGGGACTCC30.1903553299492386No Hit
TCGTCGCCTACACTCAAT30.1903553299492386No Hit
CTGGTATTGTCGTCGCCTACAC30.1903553299492386No Hit
ACCACTACGATTAGAAC30.1903553299492386No Hit
GGTATTGTCGTCGCCTACACTCA30.1903553299492386No Hit
TGTCGTCGCCTACACTCAAT30.1903553299492386No Hit
GGCCACGCGCGCTCGGGACTCCTG30.1903553299492386No Hit
GCCACGCGCGCTCGGGACT30.1903553299492386No Hit
TCCTGGTATTGTCGT30.1903553299492386No Hit
TCGTCGCCTACACTCAATTCAGTCTGTACACCAC30.1903553299492386No Hit
CGCGCTCGGGACTCCTG30.1903553299492386No Hit
GTCACGTGACCGCGA30.1903553299492386No Hit
CTGTACACCACTACGATTA30.1903553299492386No Hit
AACCACCACAGACCA30.1903553299492386No Hit
ACACTCAATTCAGTCTGTACACCAC30.1903553299492386No Hit
TTGTCGTCGCCTACACT30.1903553299492386No Hit
ACACTCAATTCAGTCTGTACACCACTA30.1903553299492386No Hit
GGACTCCTGGTATTGTCG30.1903553299492386No Hit
GACTCCTGGTATTGTCGTCGCCTACACTCAA30.1903553299492386No Hit
CGGGACTCCTGGTATTG30.1903553299492386No Hit
GGGACTCCTGGTATTGTCGTCGCCTACAC30.1903553299492386No Hit
CCACGCGCGCTCGGGAC30.1903553299492386No Hit
TCAATTCAGTCTGTACACCACTAC20.12690355329949238No Hit
TGTACACCACTACGATTAGAACGGATA20.12690355329949238No Hit
CACGCGCGCTCGGGACTC20.12690355329949238No Hit
TCGCCTACACTCAATTCAGTCTGTACACCAC20.12690355329949238No Hit
AACGGATACGGCCTTAGAGGCGTTCAGGCGGA20.12690355329949238No Hit
GTATTGTCGTCGCCTACACTCA20.12690355329949238No Hit
TACGGCCTTAGAGGCGTTCAG20.12690355329949238No Hit
TCCTGGTATTGTCGTCGCCTACACTCAATGATCG20.12690355329949238No Hit
ACACTCAATTCAGTCTGTA20.12690355329949238No Hit
AGTCTGTACACCACTAC20.12690355329949238No Hit
CCTACACTCAATTCAGT20.12690355329949238No Hit
GGCCACGCGCGCTCGGGACTCCTGGTATTGTC20.12690355329949238No Hit
CTGTACACCACTACGA20.12690355329949238No Hit
TGGTATTGTCGTCGC20.12690355329949238No Hit
AGAACGGATACGGCCTTAGA20.12690355329949238No Hit
CGCCTACACTCAATTCAGTCTGTACACCAC20.12690355329949238No Hit
GCGCTCGGGACTCCTGGTA20.12690355329949238No Hit
CACTACGATTAGAACGGATACGGCC20.12690355329949238No Hit
CAATTCAGTCTGTACAC20.12690355329949238No Hit
ATTAGAACGGATACGGCCTTAGA20.12690355329949238No Hit
TTCAGTCTGTACACCACTAC20.12690355329949238No Hit
TTGTCGTCGCCTACAC20.12690355329949238No Hit
AGGCGTTCAGGCGTA20.12690355329949238No Hit
GCCACGCGCGCTCGGGACTCCTGGTATTGT20.12690355329949238No Hit
ACACCACTACGATTAGAA20.12690355329949238No Hit
GACTCCTGGTATTGTCGTCGCCTACAC20.12690355329949238No Hit
GCGCGCTCGGGACTC20.12690355329949238No Hit
ATTGTCGTCGCCTACACTCAAGA20.12690355329949238No Hit
GCCTACACTCAATTCAGTCTGTACA20.12690355329949238No Hit
TGGTATTGTCGTCGCCTACAC20.12690355329949238No Hit
CAGTCTGTACACCACTACGAT20.12690355329949238No Hit
GCCACGCGCGCTCGGGACTCCTG20.12690355329949238No Hit
TATTGTCGTCGCCTACACTCAATT20.12690355329949238No Hit
GACTCCTGGTATTGTCGTCGCCTACACT20.12690355329949238No Hit
TACACTCAATTCAGTCTGTACACCACTACGATTA20.12690355329949238No Hit
GGCCACGCGCGCTCGGGACTCCTGG20.12690355329949238No Hit
CGCCTACACTCAATTCAGTCTGTACCCCAC20.12690355329949238No Hit
GTATTGTCGTCGCCTACACTCAAGA20.12690355329949238No Hit
GGACTCCTGGTATTGTC20.12690355329949238No Hit
GGCCACGCGCGCTCGGGACTCCTGGTATT20.12690355329949238No Hit
TATTGTCGTCGCCTACACTCAATTCA20.12690355329949238No Hit
CCTACACTCAATTCAGTCTGTACACC20.12690355329949238No Hit
GGACTCCTGGTATTGT20.12690355329949238No Hit
GACTCCTGGTATTGTCGTC20.12690355329949238No Hit
TTGTCGTCGCCTACACTCAATTCA20.12690355329949238No Hit
CCTGGTATTGTCGTCGCCTACACTCAAT20.12690355329949238No Hit
GTACACCACTACGATTA20.12690355329949238No Hit
TATTGTCGTCGCCTACACTCAATTCAG20.12690355329949238No Hit
GGTATTGTCGTCGCCTACACTCAATTC20.12690355329949238No Hit
GTCGCCTACACTCAATTCA20.12690355329949238No Hit
GGTATTGTCGTCGCCTACACTCAATTCAGG20.12690355329949238No Hit
CTCGGGACTCCTGGTATTGTCGTCGCC20.12690355329949238No Hit
CTCGGGACTCCTGGTATTG20.12690355329949238No Hit
TGGTATTGTCGTCGCC20.12690355329949238No Hit
GTATTGTCGTCGCCTACACTCAATGATC20.12690355329949238No Hit
TACACCACTACGATTAGAAC20.12690355329949238No Hit
CTGGTATTGTCGTCG20.12690355329949238No Hit
TGTCGTCGCCTACACTCAATTCA20.12690355329949238No Hit
TCCTGGTATTGTCGTCGCCTACACTCAAT20.12690355329949238No Hit
GGTATTGTCGTCGCCTACACTCAATTGAT20.12690355329949238No Hit
TCGTCGCCTACACTC20.12690355329949238No Hit
ACTCCTGGTATTGTCGT20.12690355329949238No Hit
ACTCCTGGTATTGTCGTCGCCTACACTCAAT20.12690355329949238No Hit
TCGCCTACACTCAATT20.12690355329949238No Hit
CACGCGCGCTCGGGACTCCTGGTATTGTCGTC20.12690355329949238No Hit
ATTGTCGTCGCCTACACTCAATTCAGTCTG20.12690355329949238No Hit
ATTGTCGTCGCCTACACTCAATTC20.12690355329949238No Hit
GGTATTGTCGTCGCCTACACTCAAG20.12690355329949238No Hit
GGACTCCTGGTATTGTCGTCGCCTACACTCAAT20.12690355329949238No Hit
ACTCAATTCAGTCTGTACACCACTAC20.12690355329949238No Hit
TTGTCGTCGCCTACACTCAATGAT20.12690355329949238No Hit
CCTACACTCAATTCAGTCTGTACACCA20.12690355329949238No Hit
GGGACTCCTGGTATTGTCGTCGCCTACACTCAAT20.12690355329949238No Hit
ACTCAATTCAGTCTGTACACCACTA20.12690355329949238No Hit
GGGACTCCTGGTATTGTCGT20.12690355329949238No Hit
TCGTCGCCTACACTCAATTCAGTC20.12690355329949238No Hit
GTCGCCTACACTCAAT20.12690355329949238No Hit
CACGCGCGCTCGGGACT20.12690355329949238No Hit
CGCTCGGGACTCCTGGTATTGTCGT20.12690355329949238No Hit
ACGCGCGCTCGGGACTC20.12690355329949238No Hit
ATTCAGTCTGTACACCACTACGATTA20.12690355329949238No Hit
CCTGGTATTGTCGTCGCCTACACTCAATT20.12690355329949238No Hit
ACACTCAATTCAGTCTGTACACCACTACGA20.12690355329949238No Hit
GCGCGCTCGGGACTCCTGGTA20.12690355329949238No Hit
TACACCACTACGATTAGAACGGATACGGCCT20.12690355329949238No Hit
ACGATTAGAACGGATA20.12690355329949238No Hit
CAGTCTGTACACCAC20.12690355329949238No Hit
GGTATTGTCGTCGCCTACACTCAATTCAGTCTG20.12690355329949238No Hit
CGTCGCCTACACTCA20.12690355329949238No Hit
CGCCTACACTCAATTCAGTCTG20.12690355329949238No Hit
AGTCTGTACACCACTA20.12690355329949238No Hit
ACACCACTACGATTAGAACGGATAC20.12690355329949238No Hit
GACTCCTGGTATTGTCGT20.12690355329949238No Hit
GACTCCTGGTATTGTCG20.12690355329949238No Hit
GGTATTGTCGTCGCCTAC20.12690355329949238No Hit
TCGCCTACACTCAATTC20.12690355329949238No Hit
CGTCGCCTACACTCAATTCAGTCTGTACAC20.12690355329949238No Hit
ACGGCCTTAGAGGCGTTCAGGCG20.12690355329949238No Hit
AGTCTGTACACCACTACGATTA20.12690355329949238No Hit
TCTGTACACCACTACGATTAGAACGGATA20.12690355329949238No Hit
TCACGTGACCGCGAGG20.12690355329949238No Hit
TGTCGTCGCCTACACTCA20.12690355329949238No Hit
CCTTAGAGGCGTTCA20.12690355329949238No Hit
CGCCTACACTCAATTC20.12690355329949238No Hit
TCGTCGCCTACACTCAATTCAGTCTG20.12690355329949238No Hit
GGGACTCCTGGTATTGTCGTCGCCTAC20.12690355329949238No Hit
CTCCTGGTATTGTCGTCGCCT20.12690355329949238No Hit
ATTGTCGTCGCCTACA20.12690355329949238No Hit
CCTGGTATTGTCGTCGCCTACAC20.12690355329949238No Hit
CCTGGTATTGTCGTC20.12690355329949238No Hit
CGTCGCCTACACTCAATT20.12690355329949238No Hit
CTACACTCAATTCAGTCTG20.12690355329949238No Hit
TGTCGTCGCCTACACTCAATTCAG20.12690355329949238No Hit
TCAGTCTGTACACCA20.12690355329949238No Hit
GTATTGTCGTCGCCTACACTCAATTCG20.12690355329949238No Hit
ACTCAATTCAGTCTGTACACCAC20.12690355329949238No Hit
CCACGCGCGCTCGGGACTC20.12690355329949238No Hit
GGCCACGCGCGCTCGGGACTCCTGGTA20.12690355329949238No Hit
GTACACCACTACGAT20.12690355329949238No Hit
GTATTGTCGTCGCCTACACTCAATTG20.12690355329949238No Hit
CACCACTACGATTAGAA20.12690355329949238No Hit
TACGATTAGAACGGA20.12690355329949238No Hit
CGCCTACACTCAATTCAGTCTGTACAC20.12690355329949238No Hit
GTCTGTACACCACTACGATT20.12690355329949238No Hit
GCCACGCGCGCTCGGGA20.12690355329949238No Hit
GGGACTCCTGGTATTGTCGTCGCCTACACTCAA20.12690355329949238No Hit
AACGGATACGGCCTT20.12690355329949238No Hit
AGAACGGATACGGCCTTAGAGGCGT20.12690355329949238No Hit
ACTCAATTCAGTCTGTACACCACTACGATTA20.12690355329949238No Hit
GGTATTGTCGTCGCCT20.12690355329949238No Hit
GGACTCCTGGTATTGTCGTCGCCT20.12690355329949238No Hit
CGCGCGCTCGGGACTCCTGG20.12690355329949238No Hit
ACTCCTGGTATTGTCGTCGCCTACACTCAA20.12690355329949238No Hit
TACACTCAATTCAGTCTGTACACCAC20.12690355329949238No Hit
TATTGTCGTCGCCTA20.12690355329949238No Hit
TACACCACTACGATTAGAACG20.12690355329949238No Hit
GACTCCTGGTATTGTCGTCGC20.12690355329949238No Hit
GGCCACGCGCGCTCGGGACTCCTGGTATTGT20.12690355329949238No Hit
GCCTTAGAGGCGTTCAGGCGTAATC20.12690355329949238No Hit
CACGTGACCGCGAGGGCCACGCGCG20.12690355329949238No Hit
GTATTGTCGTCGCCT20.12690355329949238No Hit
CGGGACTCCTGGTATTGTC20.12690355329949238No Hit
GACTCCTGGTATTGTCGTCGCC20.12690355329949238No Hit
GGACTCCTGGTATTG20.12690355329949238No Hit
GTACACCACTACGATTAGAACGGATACG20.12690355329949238No Hit
GAGGGCCACGCGCGC20.12690355329949238No Hit
GCGAGGGCCACGCGC20.12690355329949238No Hit
CCTACACTCAATTCAGTCTGTACACCAC20.12690355329949238No Hit
CGGCCTTAGAGGCGTTCA20.12690355329949238No Hit
GGTATTGTCGTCGCCTACACTCAATTGATC20.12690355329949238No Hit
AGAACGGATACGGCC20.12690355329949238No Hit
TCCTGGTATTGTCGTCGCCTA20.12690355329949238No Hit
CACTACGATTAGAACG20.12690355329949238No Hit
CGTCGCCTACACTCAATTCAGTCTG20.12690355329949238No Hit
CGCGCTCGGGACTCCTGG20.12690355329949238No Hit
CGTCGCCTACACTCAATTCA20.12690355329949238No Hit
TCGTCGCCTACACTCAATTCA20.12690355329949238No Hit
CGTCGCCTACACTCAATTCAG20.12690355329949238No Hit
TCAATTCAGTCTGTA20.12690355329949238No Hit
ACTCCTGGTATTGTCGTCG20.12690355329949238No Hit
CCTACACTCAATTCAG20.12690355329949238No Hit
TCGTCGCCTACACTCAATTCAG20.12690355329949238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)