FastQCFastQC Report
Fri 29 Apr 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Sobemovirus_segment_1.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614
Sequences flagged as poor quality0
Sequence length15-35
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTGTAGGCGACGACAATACC2434.328464552903456No Hit
AGTGTAGGCGACGACAATACC1442.5650160313501957No Hit
TTGAGTGTAGGCGACGACAATACC1212.1553259707873176No Hit
GAGTGTAGGCGACGACAATACC1111.9771998574991096No Hit
ATTGAGTGTAGGCGACGACAATACC1041.8525115781973638No Hit
CGAGCGCGCGTGGCC941.6743854649091556No Hit
GTAGGCGACGACAATACC881.5675097969362308No Hit
GTGTAGGCGACGACAATACC861.5318845742785894No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCC721.2825080156750979No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCC661.1756323477021733No Hit
CCCGAGCGCGCGTGGCC651.1578197363733522No Hit
CTGAATTGAGTGTAGGCGACGACAATACC540.9618810117563236No Hit
CCGAGCGCGCGTGGCC520.9262557890986819No Hit
AATTGAGTGTAGGCGACGACAATACC510.908443177769861No Hit
AGGCGACGACAATACC480.8550053437833987No Hit
TAGGCGACGACAATACC460.8193801211257571No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCC440.7837548984681154No Hit
GAATTGAGTGTAGGCGACGACAATACC440.7837548984681154No Hit
TGAGTGTAGGCGACGACAA410.730317064481653No Hit
TGTAGGCGACGACAATACC400.7125044531528322No Hit
TGAATTGAGTGTAGGCGACGACAATACC350.6234413965087282No Hit
GCGACGACAATACCA350.6234413965087282No Hit
AGTGTAGGCGACGACAA290.5165657285358034No Hit
CGACGACAATACCAG290.5165657285358034No Hit
ACTCGTTTTCAATTT280.4987531172069825No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCCC280.4987531172069825No Hit
TGAGTGTAGGCGACGACAATA280.4987531172069825No Hit
TGTAGGCGACGACAA270.4809405058781618No Hit
GTCCCGAGCGCGCGTGGCC220.3918774492340577No Hit
GAGTGTAGGCGACGACAA220.3918774492340577No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCC220.3918774492340577No Hit
GAGTCCCGAGCGCGCGTGGCC220.3918774492340577No Hit
AGTGTAGGCGACGACAATAC220.3918774492340577No Hit
TTGAGTGTAGGCGACGACAA210.3740648379052369No Hit
ACGACAATACCAGGAG210.3740648379052369No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCC200.3562522265764161No Hit
GAGTGTAGGCGACGACAATAC200.3562522265764161No Hit
AGTCCCGAGCGCGCGTGGCC200.3562522265764161No Hit
TCCCGAGCGCGCGTGGCC190.3384396152475953No Hit
CAGGAGTCCCGAGCGCGCGTGGCC190.3384396152475953No Hit
TTGAGTGTAGGCGACGACAATACCA190.3384396152475953No Hit
TTGAGTGTAGGCGACGACAATAC180.32062700391877447No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCC180.32062700391877447No Hit
ATTGAGTGTAGGCGACGACAA180.32062700391877447No Hit
GAGTGTAGGCGACGACAATACCAGGAGTCCC180.32062700391877447No Hit
GTAGGCGACGACAATAC180.32062700391877447No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCC180.32062700391877447No Hit
AGGAGTCCCGAGCGCGCGTGGCC180.32062700391877447No Hit
CCGAGCGCGCGTGGC170.3028143925899537No Hit
CCCGAGCGCGCGTGGC170.3028143925899537No Hit
GGAGTCCCGAGCGCGCGTGGCC170.3028143925899537No Hit
TGAGTGTAGGCGACGACAATAC160.28500178126113285No Hit
GTAGGCGACGACAATACCA160.28500178126113285No Hit
CGACGACAATACCAGGAGTCC160.28500178126113285No Hit
ATTGAGTGTAGGCGACGACAATAC160.28500178126113285No Hit
AGTGTAGGCGACGACAATACCAGGA160.28500178126113285No Hit
CCCGAGCGCGCGTGGCCC160.28500178126113285No Hit
GCGACGACAATACCAGGAGTCCC160.28500178126113285No Hit
AATTGAGTGTAGGCGACGACAA150.26718916993231207No Hit
TTGAGTGTAGGCGACGACAATA150.26718916993231207No Hit
GCGACGACAATACCAG150.26718916993231207No Hit
TGTAGGCGACGACAATACCA150.26718916993231207No Hit
GAATTGAGTGTAGGCGACGACAA150.26718916993231207No Hit
ACTGAATTGAGTGTAGGCGACGACAATACC150.26718916993231207No Hit
ATTGAGTGTAGGCGACGACAATACCA150.26718916993231207No Hit
CCGAGCGCGCGTGGCCC150.26718916993231207No Hit
GACTGAATTGAGTGTAGGCGACGACAATACC150.26718916993231207No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCCG140.24937655860349126No Hit
AGTGTAGGCGACGACAATACCA140.24937655860349126No Hit
GACAATACCAGGAGTCCCGAGCGCGCGTGGCC140.24937655860349126No Hit
TGAGTGTAGGCGACGACAATACCA140.24937655860349126No Hit
AGTGTAGGCGACGACAATACCAGGAGTCCC140.24937655860349126No Hit
AATTGAGTGTAGGCGACGACAATACCA140.24937655860349126No Hit
TTGAGTGTAGGCGACGACAATACCAGGAG140.24937655860349126No Hit
ACAATACCAGGAGTC130.23156394727467047No Hit
ACGACAATACCAGGA130.23156394727467047No Hit
TAGGCGACGACAATA130.23156394727467047No Hit
TAGGCGACGACAATACCA130.23156394727467047No Hit
TGAGTGTAGGCGACGACAATACCAGGA130.23156394727467047No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTC130.23156394727467047No Hit
TTACGCCTGAACGCC120.21375133594584966No Hit
AGGCGACGACAATACCAGGAGTCCC120.21375133594584966No Hit
AGGCGACGACAATACCAGGA120.21375133594584966No Hit
GGCGACGACAATACC120.21375133594584966No Hit
CGACGACAATACCAGGAGTCCC120.21375133594584966No Hit
GAGTGTAGGCGACGACAATACCA120.21375133594584966No Hit
GTAGGCGACGACAATACCAGGAGTCCC120.21375133594584966No Hit
GATTACGCCTGAACGCCT120.21375133594584966No Hit
GACGACAATACCAGGA120.21375133594584966No Hit
ACAATACCAGGAGTCCC120.21375133594584966No Hit
TTGAGTGTAGGCGACGA120.21375133594584966No Hit
AGTGTAGGCGACGACAATA120.21375133594584966No Hit
ATTACGCCTGAACGCCT110.19593872461702885No Hit
GAGTGTAGGCGACGACAATACCAGGAGTCC110.19593872461702885No Hit
ATTGAGTGTAGGCGACGACAATA110.19593872461702885No Hit
TGAGTGTAGGCGACGACAATACCAG110.19593872461702885No Hit
TGAGTGTAGGCGACGA100.17812611328820804No Hit
TGTAGGCGACGACAATACCAG100.17812611328820804No Hit
GACAATACCAGGAGTCCC100.17812611328820804No Hit
AGGCGACGACAATACCAGGAG100.17812611328820804No Hit
AGGCGACGACAATAC100.17812611328820804No Hit
GCGACGACAATACCAGGAG100.17812611328820804No Hit
AGCGCGCGTGGCCCT100.17812611328820804No Hit
GACAATACCAGGAGTCCCGAGCGCGCGTGGC100.17812611328820804No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTCCCG100.17812611328820804No Hit
TGAGTGTAGGCGACGACAATACCAGGAGT100.17812611328820804No Hit
GATTACGCCTGAACGCC100.17812611328820804No Hit
TTACGCCTGAACGCCT100.17812611328820804No Hit
TTGAGTGTAGGCGACG100.17812611328820804No Hit
AGGCGACGACAATACCAG100.17812611328820804No Hit
GACGACAATACCAGGAGTCC100.17812611328820804No Hit
CGAGCGCGCGTGGCCC90.16031350195938723No Hit
CGACGACAATACCAGGA90.16031350195938723No Hit
TGTAGGCGACGACAATAC90.16031350195938723No Hit
GTAGGCGACGACAATACCAGGA90.16031350195938723No Hit
TGAGTGTAGGCGACGAC90.16031350195938723No Hit
TGAATTGAGTGTAGGCGACGACAATACCAGGA90.16031350195938723No Hit
GACGACAATACCAGGAG90.16031350195938723No Hit
ATTACGCCTGAACGCC90.16031350195938723No Hit
TGAGTGTAGGCGACGACAAT90.16031350195938723No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGAGTCC90.16031350195938723No Hit
AGGCGACGACAATACCAGGAGTCC90.16031350195938723No Hit
TGAATTGAGTGTAGGCGACGACAA90.16031350195938723No Hit
GAGCGCGCGTGGCCC80.14250089063056642No Hit
TGTAGGCGACGACAATA80.14250089063056642No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAATACC80.14250089063056642No Hit
GTGTAGGCGACGACAATAC80.14250089063056642No Hit
ACGACAATACCAGGAGTCCC80.14250089063056642No Hit
GTGTAGGCGACGACAA80.14250089063056642No Hit
GAATTGAGTGTAGGCGACGACAATACCAG80.14250089063056642No Hit
ACAGACTGAATTGAGTGTAGGCGACGACAA80.14250089063056642No Hit
GTGTAGGCGACGACAATACCAGGAGTCCC80.14250089063056642No Hit
AGGCGACGACAATACCA80.14250089063056642No Hit
ATTGAGTGTAGGCGACGACAATACCAG80.14250089063056642No Hit
GTGTAGGCGACGACAAT80.14250089063056642No Hit
GGAGTCCCGAGCGCGCGTGGC80.14250089063056642No Hit
TTGAGTGTAGGCGACGACA80.14250089063056642No Hit
ATTGAGTGTAGGCGACGACAATACCAGGA80.14250089063056642No Hit
GTAGGCGACGACAATACCAGGAG80.14250089063056642No Hit
AATACCAGGAGTCCC80.14250089063056642No Hit
AGCGCGCGTGGCCCTC80.14250089063056642No Hit
GACGACAATACCAGGAGTCCCGAGCGCGCGTGGCC80.14250089063056642No Hit
ACGCCTGAACGCCTC80.14250089063056642No Hit
GAGTGTAGGCGACGACAATA80.14250089063056642No Hit
AATTGAGTGTAGGCGACGACAATACCAGGA80.14250089063056642No Hit
TGAGTGTAGGCGACG80.14250089063056642No Hit
GCGACGACAATACCAGGA80.14250089063056642No Hit
GACGACAATACCAGGAGTC80.14250089063056642No Hit
TAGGCGACGACAATAC80.14250089063056642No Hit
GACAATACCAGGAGTC80.14250089063056642No Hit
GAATTGAGTGTAGGCGACGACAATACCAGGA80.14250089063056642No Hit
ACGACAATACCAGGAGTCCCGAGCGCGCGTGGCC80.14250089063056642No Hit
GTCCCGAGCGCGCGTGGC70.12468827930174563No Hit
AGTGTAGGCGACGACAATACCAGGAGTCCCG70.12468827930174563No Hit
TTGAGTGTAGGCGACGACAATACCAG70.12468827930174563No Hit
ATTGAGTGTAGGCGACGACAATACCAGGAGTC70.12468827930174563No Hit
TAGGCGACGACAATACCAGGA70.12468827930174563No Hit
ATACCAGGAGTCCCGAGCGCGCGTGGC70.12468827930174563No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTC70.12468827930174563No Hit
TGAGTGTAGGCGACGACAATACCAGG70.12468827930174563No Hit
TTACGCCTGAACGCCTC70.12468827930174563No Hit
AGGAGTCCCGAGCGCGCGTGGC70.12468827930174563No Hit
TCCCGAGCGCGCGTGGC70.12468827930174563No Hit
TGTAGGCGACGACAATACCAGGA70.12468827930174563No Hit
ACGACAATACCAGGAGTC60.10687566797292483No Hit
ACCAGGAGTCCCGAGCGCGCG60.10687566797292483No Hit
TGAGTGTAGGCGACGACA60.10687566797292483No Hit
GTAGGCGACGACAATA60.10687566797292483No Hit
TGAGTGTAGGCGACGACAATACCAGGAG60.10687566797292483No Hit
GACGACAATACCAGGAGTCCC60.10687566797292483No Hit
GTCCCGAGCGCGCGTGGCCC60.10687566797292483No Hit
GTGTAGGCGACGACAATACCAGGAGTC60.10687566797292483No Hit
GCGACGACAATACCAGGAGTCCCGA60.10687566797292483No Hit
TGAGTGTAGGCGACGACAATACCAGGAGTCCCG60.10687566797292483No Hit
ACCAGGAGTCCCGAGCGCGCGTGGCC60.10687566797292483No Hit
CGACGACAATACCAGGAG60.10687566797292483No Hit
TACGCCTGAACGCCT60.10687566797292483No Hit
GACGACAATACCAGGAGT60.10687566797292483No Hit
CTGAATTGAGTGTAGGCGACGACAA60.10687566797292483No Hit
AGTGTAGGCGACGACAATACCAGGAGTC60.10687566797292483No Hit
TGTAGGCGACGACAATACCAGGAGTCCC60.10687566797292483No Hit
AATTGAGTGTAGGCGACGA60.10687566797292483No Hit
TGAATTGAGTGTAGGCGACGACAATAC60.10687566797292483No Hit
AATTGAGTGTAGGCGACGACAATA60.10687566797292483No Hit
CCAGGAGTCCCGAGCGCGCGTGGC60.10687566797292483No Hit
CGACGACAATACCAGGAGTCCCGAG60.10687566797292483No Hit
TTGAGTGTAGGCGACGACAATACCAGGAGTCCCGA60.10687566797292483No Hit
GAGTGTAGGCGACGACAATACCAG60.10687566797292483No Hit
ACAATACCAGGAGTCCCGAGCGCGCGTGGCC60.10687566797292483No Hit
CCAGGAGTCCCGAGCGCGCGTGGCC60.10687566797292483No Hit
ACAATACCAGGAGTCCCGAGC60.10687566797292483No Hit
TCCCGAGCGCGCGTGGCCC60.10687566797292483No Hit
ATTGAGTGTAGGCGACG60.10687566797292483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)