FastQCFastQC Report
Fri 29 Apr 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089280
Sequences flagged as poor quality0
Sequence length15-35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACGGAATTCTTGAACCAAGCGCAAGCGG168360.5449813548788067No Hit
AATCACGGAATTCTTGAACCAAGCGCAAGCGG61510.19910788274290447No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG54150.17528356121814792No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGGG48010.15540837994613632No Hit
AACCGCCACGGACGAAA44250.1432372591671846No Hit
GGGGGGGGGGGGGGGGGGGGGGGG41500.13433550859747254No Hit
GGGGGGGGGGGGGGGGGGGG39880.12909156826186036No Hit
ATCACGGAATTCTTGAACCAA39070.12646959809405428No Hit
GGGGGGGGGGGGGGG38990.12621063807748084No Hit
GGGGGGGGGGGGGGGGGGG38750.12543375802776052No Hit
GGGGGGGGGGGGGGGGG38450.12446265796561011No Hit
GGGGGGGGGGGGGGGGGGGGGG38260.12384762792624819No Hit
GGGGGGGGGGGGGGGGGG37620.12177594779366066No Hit
GGGGGGGGGGGGGGGGGGGGG37570.12161409778330226No Hit
AACCGCCACGGACGAAAG36550.11831235757199089No Hit
GGGGGGGGGGGGGGGG36010.11656437746012016No Hit
ATCACGGAATTCTTGAACCAAGCGCAA31360.10151232649678889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)