FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Ambiguivirus.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Ambiguivirus.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177
Sequences flagged as poor quality0
Sequence length15-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGAGTGGTACTGCATTTGAG42.2598870056497176No Hit
CCGGCACCCACTGTACCTG42.2598870056497176No Hit
TTCCAGTCAGTTTGCCTAGCCATCTCCGGTCACG42.2598870056497176No Hit
TTGCTCAGAGCGATCGTTGCGAT31.694915254237288No Hit
TGACAGGGCGGGCCGAGG31.694915254237288No Hit
TCCAGTCAGTTTGCCTAGCCATCTCCGGTCACG31.694915254237288No Hit
TTAAGTCCGTAGCCTCTGGGA21.1299435028248588No Hit
AGCGCTGGATTGGGCCCGACGTTCTGGACT21.1299435028248588No Hit
TCCAGTGCGTGAGCTAGAACGCGA21.1299435028248588No Hit
CGGCCAAAGATGGAGCTACCTTGACACTGCGA21.1299435028248588No Hit
CCAGGACGGCCAGGC21.1299435028248588No Hit
TTTTGGTCTGGGGTTTATGT21.1299435028248588No Hit
GCGGTTGTAGCACCTGAGCTTGTCT21.1299435028248588No Hit
CGGAGTGGCGGACCG21.1299435028248588No Hit
TGCAGCGGTTGTAGCACCTGAGC21.1299435028248588No Hit
GGCGAGTGTGTAACCTGCTTCCCGGA21.1299435028248588No Hit
AGATGCAGGCTGCGTCGGAGGTCGAAGTGGGTT21.1299435028248588No Hit
TGCAAACCTCGGTCGCTAGTTATT21.1299435028248588No Hit
TTGCTGGACGGAGAAGTTGGAG21.1299435028248588No Hit
TCGAAAGCGCTGGATTGGGCCCG21.1299435028248588No Hit
GGTTCTTCCGGTGTCGGAAAGTCGAGGGTTGTC21.1299435028248588No Hit
AGCGGTTGTAGCACCTGAGCTTGTC21.1299435028248588No Hit
CGGAAAGTGCGGAAGTCGTCTGGTGCGGGA21.1299435028248588No Hit
TTGCTGGGCACTTTGCGTTTTGGGTCTTAGT21.1299435028248588No Hit
CGGAAGTCGTCTGGTGCGGGCCTAGGGTCGGGA10.5649717514124294No Hit
TCCGGAGGGACCTCTTCTTATGCC10.5649717514124294No Hit
TCTGAACGGGCCCCA10.5649717514124294No Hit
CAGAGCGATCGTTGCGATTTTGGGTGGGT10.5649717514124294No Hit
AGTCGAGGGTTGTCGCTAAAGGTCTGAACGGGC10.5649717514124294No Hit
TGGGCGTGTCGGGCCTTTGCGG10.5649717514124294No Hit
CGGGAGAGCTTCGCC10.5649717514124294No Hit
TCGAGGGTTGTCGCTAAAGGT10.5649717514124294No Hit
CGAAAGCGCTGGATTGGGCCCGACGTTCTGGACT10.5649717514124294No Hit
CGCGGAGTGGCGGACCGTGACGTCACGCATCTG10.5649717514124294No Hit
ACGGGCCCCAGCGTGCTGCTTT10.5649717514124294No Hit
TTTGCTGGACGGAGAAGTTGGAGT10.5649717514124294No Hit
TGGCAACTGGAGCAAGAACACGC10.5649717514124294No Hit
TAAGGCATTCGGCGGAAAGTGC10.5649717514124294No Hit
TGATGGTGCGTGATTGGGTCAAGG10.5649717514124294No Hit
TTCTTCTTTCCTTGCCTTGTCTTTCTTTCTGGCT10.5649717514124294No Hit
TACCTTGAGGCGGAACGTTCTC10.5649717514124294No Hit
TCGTTGCGATTTTGGGTGGGT10.5649717514124294No Hit
GTTCCGTTTGTTGAGCGCGGTGCGGACTCACCCG10.5649717514124294No Hit
TAAACTGGGGCGTCACGATTAGCAGGG10.5649717514124294No Hit
CGGTTGTAGCACCTGAGCTTGTCT10.5649717514124294No Hit
TTGAGTCGTCAGTGTTGTCACC10.5649717514124294No Hit
GGGAAGATGGTCCTTTGTGC10.5649717514124294No Hit
GAGGTGGACAAGTGG10.5649717514124294No Hit
GGTTGGTTCTTCCGGTGTCGGAAAGTCGAGGGT10.5649717514124294No Hit
TGCAGCTCTTCCTTTTCGTTAGC10.5649717514124294No Hit
TGGCAACTGGAGCAAGAACACG10.5649717514124294No Hit
CAAGTGGCAACTGGAGCAAGAACA10.5649717514124294No Hit
CTAGATGCAGGCTGCGTCGGAGGTCGAAGTGGGTT10.5649717514124294No Hit
TACTCTCCGGAGGGACCTCTTA10.5649717514124294No Hit
CAGGGGCGGGGACCCGCTGGATCTTGCGCT10.5649717514124294No Hit
TACCTTGACACTGCGAACTCTTAC10.5649717514124294No Hit
AGCGCTGGATTGGGCCCGACGTTCTGGAC10.5649717514124294No Hit
TTGCGGTGGTCCTGGGGAAAAC10.5649717514124294No Hit
GCGGCCAAAGATGGAGCTACCTTGACACTGC10.5649717514124294No Hit
TTGCACATGCGCGGCAGGACAAGC10.5649717514124294No Hit
CTAGAACGCGTCCGGTTTGGGCAGTGTGCGCCT10.5649717514124294No Hit
TTCACTTGCTGTGGCCCTCCA10.5649717514124294No Hit
TCGAGGGTTGTCGCTAAAGGTCTGA10.5649717514124294No Hit
TGTGGGCCCAACGAGTTCTGCAAC10.5649717514124294No Hit
TACCCCGGCCGAGCAGAAACTTTATGA10.5649717514124294No Hit
TGTGGACTGGATTTCAAGCGTGCGG10.5649717514124294No Hit
TCTGCAACAATCTTCTTCCAGGCGTTCGGTGGTG10.5649717514124294No Hit
GTTCCAGTCAGTTTGCCTAGCCATCTCCGGTCACG10.5649717514124294No Hit
TAAGTCCGTAGCCTCTGGGATGGG10.5649717514124294No Hit
CGGAAGTCGTCTGGTGCGGGC10.5649717514124294No Hit
TTAAGTCCGTAGCCTCTGGGATG10.5649717514124294No Hit
AGCGGTTGTAGCACCTGAGCTTGTCT10.5649717514124294No Hit
TTGTAAGGCATTCGGCGGAAAGT10.5649717514124294No Hit
TTCGAGCTTTGGTTAGGACG10.5649717514124294No Hit
AAACGCGTTCTCGCTCCAACACCAGGGTCGGCTG10.5649717514124294No Hit
GCGCGCTGTGCTTGGTGGTTCGGGGCAAGTT10.5649717514124294No Hit
TTTGGTGTTACCCCGGCCGAGC10.5649717514124294No Hit
CGCTGTCTACGACGCTGCCTTTCGCTCTGA10.5649717514124294No Hit
CCGCTGCTGCTGCTCTGGCTTTGACTGAGGA10.5649717514124294No Hit
AACACGCTGTCTACGACGCTGCCTTTCGCTCT10.5649717514124294No Hit
ACCTGGCCGACGGTGGATTGTC10.5649717514124294No Hit
TGCGCCTGTTCGGACCACCCGTGGT10.5649717514124294No Hit
TTGGGCCCGACGTTCTGGACTGTCATGG10.5649717514124294No Hit
TGGGATGGGCGTGTCGGGCCT10.5649717514124294No Hit
TTCGGTGAAATTCGCCCTTGTGCGTTGGG10.5649717514124294No Hit
GCTGTCTACGACGCTGCCTTTCGCTCTGA10.5649717514124294No Hit
TAGAACGCGTCCGGTTTGGG10.5649717514124294No Hit
TACCTTGACACTGCGAACTCTTACACTGGTGCTC10.5649717514124294No Hit
TTTAAACTGGGGCGTCACGAT10.5649717514124294No Hit
TGAGCGCGGTGCGGACTCACCC10.5649717514124294No Hit
TCATGGTGAATCTGTGCGGGC10.5649717514124294No Hit
AAGTGGCAACTGGAGCA10.5649717514124294No Hit
CTAGAACGCGTCCGGTTTGGGCA10.5649717514124294No Hit
AGGAGAATGACGACGGCGTGGAC10.5649717514124294No Hit
GTTGAGCGCGGTGCGGACTCACCCGGC10.5649717514124294No Hit
GTGTTACCCCGGCCGAGCAGAAACT10.5649717514124294No Hit
CGCTTTGCGGTGGTC10.5649717514124294No Hit
CTCCGGAGGGACCTCTTC10.5649717514124294No Hit
TTTGTGCGCGCTGTGCTTGGT10.5649717514124294No Hit
GTGTTGTCACCTCTGTTCTTCTTTCCTTGCCT10.5649717514124294No Hit
CAAACCTCGGTCGCTA10.5649717514124294No Hit
GTTGCCCCATACATCAAGGACGGGGAGGAGGGTC10.5649717514124294No Hit
TTGGGCCCGACGTTCTGGACTGT10.5649717514124294No Hit
TTCGGGCCCCAGCGTG10.5649717514124294No Hit
TCTGCAACAATCTTCTTCCAGGCGTTCGGTGGTGT10.5649717514124294No Hit
ACTCTCCGGAGGGACCTCTT10.5649717514124294No Hit
AGAAGTCGTCTGGTGCGGGC10.5649717514124294No Hit
TACGACGCTGCCTTTCGCTCTGACGAG10.5649717514124294No Hit
ACACGCTGTCTACGACGCTGCCTTTCGCTCTG10.5649717514124294No Hit
GACGAGTGGTACTGC10.5649717514124294No Hit
TTGGGCCCGACGTTCTGGACTGTCA10.5649717514124294No Hit
GGCCGTCGGGTGGGCG10.5649717514124294No Hit
CGTTTGCTGGGCACTTTGCGTTTTGGGTCT10.5649717514124294No Hit
CTTCCAGGCGTTCGGTGGTGTTGGCAC10.5649717514124294No Hit
TTTGTGCGCGCTGTGCTTGGTG10.5649717514124294No Hit
TCATGGTCCATGTTCTCGTTCG10.5649717514124294No Hit
CCTTGACACTGCGAACTC10.5649717514124294No Hit
TTGGGCCACCGCTTCGGCCGTGG10.5649717514124294No Hit
TGCACATGCGCGGCAGGACAAGCTGTGGACTGGA10.5649717514124294No Hit
AGAACGCGTCCGGTTTGGGCAG10.5649717514124294No Hit
ATGACAGGAGGCACTTGGGCTTAACTG10.5649717514124294No Hit
CGAGCTGAAGCGTTTGTTGGGCAAG10.5649717514124294No Hit
ATGACGACGGCGTGGACGCCACCTTTGCCAGG10.5649717514124294No Hit
CCTGCTTCCCGGATTTGGTG10.5649717514124294No Hit
CAGGGGCGGGGACCCGCTGGATCTTGCGCTTTTG10.5649717514124294No Hit
TTGGAGAGTCCAGTGCGTGAGCTAGAACG10.5649717514124294No Hit
TTGTAAGGCATTCGGCGGAAAGTGCGGAA10.5649717514124294No Hit
CGCTGTCTACGACGCTGCC10.5649717514124294No Hit
TTGTCTTCCTCGTTTGCTGGGC10.5649717514124294No Hit
TGTGGACTGGATTTCAAGCGTGC10.5649717514124294No Hit
ACCCTACCTGCATTTGCAACTCGCCT10.5649717514124294No Hit
TCTGAACGGGCCCCAGCGTGCTGC10.5649717514124294No Hit
TGCAAACCTCGGTCGCTAGTTAT10.5649717514124294No Hit
CTCGTTTGCTGGGCACTTTGCGTTTTGGG10.5649717514124294No Hit
GGCTGGGGAGCAGCAGTTGGTGAGGCTGGGGGTG10.5649717514124294No Hit
GTTGTCACCTCTGTTCTTCTTTCCTTGCCTTG10.5649717514124294No Hit
TTTGTTGAGCGCGGTGCGGACTCACC10.5649717514124294No Hit
ATGACAGGAGGCACTTGGGCTT10.5649717514124294No Hit
CATCAAGGACGGGGAGGAGGGTCAAG10.5649717514124294No Hit
GTCGTGGCGCAGGGGGGGCC10.5649717514124294No Hit
GTTCCAGTCAGTTTGCCTAGCCATCTCCG10.5649717514124294No Hit
TGCGCCTGTTCGGACCACCC10.5649717514124294No Hit
TAGGCGTCCTGTACTTCAGAGC10.5649717514124294No Hit
TACTGCATTTGAGTCGTCTGTG10.5649717514124294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACTG50.061.00000421
Produced by FastQC (version 0.11.5)