FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_1.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_1.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67688
Sequences flagged as poor quality0
Sequence length15-35
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTCGGGGTAACCCGAAAAGACCCGG43166.376314856399953No Hit
TTTCGTGGCCCTCGTTTCC25313.7392152227869047No Hit
GTTTCGTGGCCCTCG23523.47476657605484No Hit
TTTCGTGGCCCTCGTTTCCG21203.1320174920222197No Hit
GTTTCGTGGCCCTCGTTTCC18712.764153173383761No Hit
GTTTCGTGGCCCTCGTTTCCG15392.273667415199149No Hit
TGTTTCGTGGCCCTCG13301.9648977662214868No Hit
TTTCGTGGCCCTCGTTTCCGATC13131.9397825316156483No Hit
TGTTTCGTGGCCCTC12201.8023874246542961No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGA11971.7684079895993383No Hit
GTTTCGTGGCCCTCGTTTCCGATC11771.7388606547689398No Hit
TGTTTCGTGGCCCTCGTTTCC10781.5926013473584681No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCG9921.4655478075877555No Hit
ATGTTTCGTGGCCCTC9721.4360004727573574No Hit
ATGTTTCGTGGCCCTCG8401.2409880628767285No Hit
TTTCGTGGCCCTCGTTTC7801.1523460583855336No Hit
TTTCGTGGCCCTCGT7731.1420044911948943No Hit
TGTTTCGTGGCCCTCGTTTCCG7251.0710908876019383No Hit
TGTTTCGTGGCCCTCGTTTCCGATC6931.023815151873301No Hit
GTTTCGTGGCCCTCGT6150.9085805460347476No Hit
GTTTCGTGGCCCTCGTTTC5060.747547571209077No Hit
GTTTCGTGGCCCTCGTTTCCGA4880.7209549698617185No Hit
TGTTTCGTGGCCCTCGT4840.7150455028956388No Hit
CCGGAGAAGCTGAGGGGAGCTC4840.7150455028956388No Hit
TTCGTGGCCCTCGTTTCCG4810.7106134026710791No Hit
TGTTTCGTGGCCCTCGTTTC4540.6707245006500414No Hit
TGTTTCGTGGCCCTCGTTTCCGA4400.6500413662687625No Hit
GTTTCGTGGCCCTCGTTTCCGAT4260.6293582318874837No Hit
TTTCGTGGCCCTCGTTTCCGA4230.624926131662924No Hit
TTCGTGGCCCTCGTTTCC4200.6204940314383642No Hit
CCTCGTCGGGGTAACCCGA3800.5613993617775677No Hit
CCCGGAGAAGCTGAGGGGAGCTC3730.5510577945869283No Hit
AGGCCTCGTCGGGGTAACCC3630.5362841271717291No Hit
TTTCGTGGCCCTCGTTTCCGAT3460.5111688925658906No Hit
CCTCGTCGGGGTAACCCGAAAAGACCC3370.49787259189221134No Hit
CGGAGAAGCTGAGGGGAGCTC3160.4668478903202931No Hit
TTTCGTGGCCCTCGTT3120.4609384233542135No Hit
ATGTTTCGTGGCCCT3040.4491194894220541No Hit
GTTTCGTGGCCCTCGTT2900.4284363550407754No Hit
CATGTTTCGTGGCCCTC2680.3959342867273372No Hit
AGGCCTCGTCGGGGTAACCCGA2670.3944569199858173No Hit
CTCGTCGGGGTAACCCGAAAAGACCCGG2650.39150218650277746No Hit
ATGTTTCGTGGCCCTCGTTTCCG2630.38854745301973764No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCG2600.38411535279517783No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCGA2520.37229641886301856No Hit
CATGTTTCGTGGCCCTCG2500.3693416853799787No Hit
TGTTTCGTGGCCCTCGTTTCCGAT2450.3619548516723792No Hit
ATGTTTCGTGGCCCTCGTTTCC2420.3575227514478194No Hit
CCGGAGAAGCTGAGGGGAGCT2300.3397943505495804No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCGA2280.3368396170665406No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCGATCTG2240.33093015010046095No Hit
GAAGGCCTCGTCGGG2150.3176338494267817No Hit
TCGTGGCCCTCGTTTCCG2080.3072922822361423No Hit
TTCGTGGCCCTCGTTTC2050.30286018201158255No Hit
AAAGACCCGGAGAAGCTGAGGGGAGCTCCGG2010.29695071504550286No Hit
AGGCCTCGTCGGGGTAACCCGAAA1970.2910412480794232No Hit
AGGCCTCGTCGGGGTAACCCGAA1970.2910412480794232No Hit
TTTCGTGGCCCTCGTTTCCGATCT1890.2792223141472639No Hit
GAAGGCCTCGTCGGGGTAACCCGAAA1880.277744947405744No Hit
AGGGGAGCTCCGGGG1880.277744947405744No Hit
TTCGTGGCCCTCGTTTCCGATCTTTGCGATCTG1880.277744947405744No Hit
ATCTGTGTCGCGAAGG1870.2762675806642241No Hit
ATGTTTCGTGGCCCTCGTTTCCGATC1850.27331284718118426No Hit
CCGGAGAAGCTGAGGGGAGCTCC1840.2718354804396644No Hit
ATGTTTCGTGGCCCTCGT1810.26740338021510457No Hit
TCGTGGCCCTCGTTTCC1790.26444864673206475No Hit
TTCGTGGCCCTCGTTTCCGATC1790.26444864673206475No Hit
CGTGGCCCTCGTTTCCG1790.26444864673206475No Hit
CATGTTTCGTGGCCC1720.25410707954142536No Hit
TGTTTCGTGGCCCTCGTT1690.2496749793168656No Hit
TTTCGTGGCCCTCGTTTCCGATCTTTGCGATCTGT1680.2481976125753457No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCG1660.24524287909230588No Hit
CCTCGTCGGGGTAACC1660.24524287909230588No Hit
GATCTGTGTCGCGAAGG1660.24524287909230588No Hit
GTTTCGTGGCCCTCGTTT1630.24081077886774616No Hit
GTGGCCCTCGTTTCCG1620.2393334121262262No Hit
CGGAGAAGCTGAGGGGAGCT1590.23490131190166647No Hit
CCGCTCTCATGTTTCGTGGCCC1570.23194657841862665No Hit
TGAGGGGAGCTCCGGGG1570.23194657841862665No Hit
AAGGCCTCGTCGGGGTAACCCGA1520.22455974471102705No Hit
TGTTTCGTGGCCCTCGTTTCCGATCTTTGCGA1500.22160501122798723No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAGAAGC1450.2142181775203877No Hit
CGGAGAAGCTGAGGGGAGCTCC1400.2068313438127881No Hit
TGTTTCGTGGCCCTCGTTTCCGATCTTTGCG1390.20535397707126818No Hit
GAGAAGCTGAGGGGAGCTC1340.1979671433636686No Hit
GGAGAAGCTGAGGGGAGCTC1300.19205767639758892No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAG1290.190580309656069No Hit
TCTGTGTCGCGAAGGC1290.190580309656069No Hit
TCGTGGCCCTCGTTTCCGATC1280.1891029429145491No Hit
CTCGTCGGGGTAACCCGAAAAGACCCGGA1270.1876255761730292No Hit
ATGTTTCGTGGCCCTCGTTTCCGA1260.18614820943150928No Hit
GTTTCGTGGCCCTCGTTTCCGATCT1240.18319347594846944No Hit
AAGGCCTCGTCGGGGTAACCCGAAA1230.18171610920694953No Hit
ATGTTTCGTGGCCCTCGTTTC1220.18023874246542962No Hit
CGTGGCCCTCGTTTCC1220.18023874246542962No Hit
CATGTTTCGTGGCCCTCGTTTCCG1200.17728400898238977No Hit
CGCGAAGGCCTCGTCGGGGTAACCCGAAA1180.17432927549934996No Hit
AAAGACCCGGAGAAGCTGA1170.17285190875783005No Hit
GGCCCTCGTTTCCGATCTTTGCG1170.17285190875783005No Hit
CCTCGTCGGGGTAACCCGAAAAGACC1160.17137454201631014No Hit
GCCTCGTCGGGGTAACCCGAAAAGACCCGG1150.1698971752747902No Hit
AAAGACCCGGAGAAGCTGAGGGGAGCTC1150.1698971752747902No Hit
AAGGCCTCGTCGGGGTAACCCGAA1140.1684198085332703No Hit
AAGGCCTCGTCGGGGTAACCC1130.16694244179175038No Hit
CTCATGTTTCGTGGCCCTC1130.16694244179175038No Hit
CCTCGTCGGGGTAACCC1130.16694244179175038No Hit
TCTGTGTCGCGAAGG1120.16546507505023048No Hit
CATGTTTCGTGGCCCTCGTTTCC1120.16546507505023048No Hit
TTTCGTGGCCCTCGTTTCCGATCTTT1120.16546507505023048No Hit
TTTCGTGGCCCTCGTTT1050.15512350785959106No Hit
CGAAGGCCTCGTCGGGGTAACCCGAAA1040.15364614111807115No Hit
GAAGGCCTCGTCGGGGTAACCCGAA1040.15364614111807115No Hit
TCATGTTTCGTGGCCCTC1040.15364614111807115No Hit
GAAGGCCTCGTCGGGGTAACCCGA1030.15216877437655124No Hit
CTGAGGGGAGCTCCGGGG990.14625930741047158No Hit
CGTGGCCCTCGTTTCCGATC980.14478194066895167No Hit
GTGGCCCTCGTTTCCGATC970.14330457392743176No Hit
CGCTCTCATGTTTCGTGGCCC970.14330457392743176No Hit
ATGTTTCGTGGCCCTCGTTTCCGAT960.14182720718591182No Hit
AGGCCTCGTCGGGGTAACC950.14034984044439192No Hit
AAAAGACCCGGAGAAGCTGAGGGGAGCTCCGG930.1373951069613521No Hit
CCCGGAGAAGCTGAGGGGAGCTCC930.1373951069613521No Hit
ACCGCTCTCATGTTTCGTGGCCCT920.1359177402198322No Hit
TGTTTCGTGGCCCTCGTTT900.13296300673679234No Hit
ATCTGTGTCGCGAAG890.13148563999527244No Hit
AAGACCCGGAGAAGCTGAGGGGAGCTCCGG880.13000827325375253No Hit
AAAGACCCGGAGAAGCTGAGGGGAGCTCCG880.13000827325375253No Hit
AGACCCGGAGAAGCTGAGGGGAGCTC870.1285309065122326No Hit
GAAAAGACCCGGAGAAGCTGAGGGGAGCTC860.12705353977071268No Hit
TCGTGGCCCTCGTTTC850.12557617302919277No Hit
CTCATGTTTCGTGGCCC830.12262143954615294No Hit
CTCGTCGGGGTAACCCGA820.12114407280463303No Hit
GTGGCCCTCGTTTCCGATCTTTGCGATCTG810.1196667060631131No Hit
CTCATGTTTCGTGGCCCTCGTTTCCGATC810.1196667060631131No Hit
GTTTCGTGGCCCTCGTTTCCGATCTTTGCGAT810.1196667060631131No Hit
CGGAGAAGCTGAGGGGAGC790.11671197258007328No Hit
ATCTGTGTCGCGAAGGCCTCGTCGGGGTAACCCGA780.11523460583855337No Hit
AAGACCCGGAGAAGCTGAGGGGAGCTC780.11523460583855337No Hit
ACCGCTCTCATGTTTCGTGGCCC770.11375723909703346No Hit
AAAGACCCGGAGAAGCTG770.11375723909703346No Hit
CTCTCATGTTTCGTGGCCC770.11375723909703346No Hit
CGTCGGGGTAACCCGAAAAGACCCGGAGAAGCTG760.11227987235551352No Hit
GGGGTAACCCGAAAAGACCCGGAGAAGCTGAG760.11227987235551352No Hit
CATGTTTCGTGGCCCT750.11080250561399362No Hit
CTCGTCGGGGTAACC750.11080250561399362No Hit
TCATGTTTCGTGGCCCTCG750.11080250561399362No Hit
TCATGTTTCGTGGCCC740.1093251388724737No Hit
CGCGAAGGCCTCGTCGGGGTAACCCGAA740.1093251388724737No Hit
CATGTTTCGTGGCCCTCGTTTCCGATC740.1093251388724737No Hit
CCTCGTCGGGGTAACCCGAAAAGACCCGGAGAA730.1078477721309538No Hit
GGGGTAACCCGAAAAGACCCGGAGAAGCTGA730.1078477721309538No Hit
CGCTCTCATGTTTCGTGGCCCTCGTTTCCGATC720.10637040538943388No Hit
GGGTAACCCGAAAAGACCCGGAGAAGCTG700.10341567190639404No Hit
TTCGTGGCCCTCGTTTCCGAT700.10341567190639404No Hit
AGACCCGGAGAAGCTGAGGGGAGCTCCGG680.10046093842335421No Hit
TTCGTGGCCCTCGTT680.10046093842335421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG2150.0160.7370328
GATCTGT1352.2874332E-5146.81729
GCGATCT2100.0105.2131127
TGCGATC2350.080.5510526
TTGCGAT2650.057.95968625
ACCCGGA4650.048.3075324
GACCCGG9900.048.2528723
AGACCCG11150.043.89393222
AAGACCC11450.042.6262421
AAAGACC11350.040.8888920
AAAAGAC10650.040.23195319
ATCTGTG1802.8696668E-636.70424729
GAAAAGA10400.035.99839818
TTTGCGA4000.035.46789624
CGAAAAG10250.030.91532117
CCGAAAA10250.027.43448616
CTTTGCG5300.023.54612425
CCCGAAA11200.022.29921215
AACCCGA13400.019.72168529
ACCCGAA12050.018.15316214
Produced by FastQC (version 0.11.5)