FastQCFastQC Report
Sun 15 May 2022
S38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS38_SantoTomePrincipe.sRNAs.15-35nt.Partitivirus_1_segment_2.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length15-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCACGTTCGTGG418.181818181818183No Hit
TGAGCCAGGTAAGATTTCCG29.090909090909092No Hit
TCATTAAAGAACGGACCAGTT29.090909090909092No Hit
TAGCGGAAACAGTGATTGCCATTC29.090909090909092No Hit
TAAAAAAACGGACAATCCCCATGGA14.545454545454546No Hit
TCGAGAAAACGACGGGCTGG14.545454545454546No Hit
TAGCAGGAATGAGCGGTACACG14.545454545454546No Hit
TCCAATCAAAGCCCAGGTCGTCA14.545454545454546No Hit
TTCTCAGTGATGACGACGGCT14.545454545454546No Hit
TTCTCAGTGATGACGACGGCTGCAC14.545454545454546No Hit
TAGAGGACGGGTGAAAAGAATTCAGGAACCA14.545454545454546No Hit
TTTCGTTGGGTCTCAAGGTTAAT14.545454545454546No Hit
TAACGGTTGCAGCAGTTGAG14.545454545454546No Hit
TTGGAATAGCCGTGAGAGGACA14.545454545454546No Hit
TGCCAGAAGCGTTCAGTGCTG14.545454545454546No Hit
CAATCGGGTTAGAACATCAGT14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)